脂联素对TNF_医学论文.docVIP

脂联素对TNF_医学论文 脂联素对TNF_医学论文 作者：刘金波，邓华聪，葛倩，李丙蓉，郑宏庭，兰丽珍 【关键词】 脂联素；肿瘤坏死因子α；内皮,血管/细胞学；动脉硬化 　　Influence of adiponectin on vascular inflammatory reaction mediated by TNFα 　　LIU JinBo, DENG HuaCong, GE Qian, LI BingRong, ZHENG HongTing, LAN LiZhen 　　Department of Endocrinology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China 　　【Abstract】 AIM: To investigate the influence of adiponectin on the inflammatory reaction in vascular endothelial cells induced by TNFα. METHODS: Human umbilical vein endothelial cells(HUVEC) were cultured in vitro and divided into normal group, TNFα stimulation group and adiponectin and TNFα associated stimulation group. HUVEC were preincubated with human recombinant adiponectin for 30 min to 16 h, then exposed to human recombinant TNFα for 6 to 12 h (adiponectin and TNFα associated stimulation group), which was then compared with TNFα stimulation group in the expressions of NO, iNOS, ET1 and MCP1. The interaction between adiponectin and HUVEC was estimated by chemical method, radioimmunoassay and ELISA. RESULTS: The expressions of NO, iNOS, ET1 and MCP1 were increased in HUVEC stimulated by TNFα for 6 to 12 h (607.7±7.6, 42.2±2.2, 199.3±11.9, 167.7±15.9 vs 543.5±2.2, 23.8±2.0, 148.4±5.3, 128.0±8.4 0.05). Compared with TNFα stimulation group, the expressions of NO, iNOS, ET1 and MCP1 in adiponectin and TNFα associated stimulation group were decreased (565.7±13.0, 36.5±2.7, 170.8±8.3, 142.6±5.6 vs 607.7±7.6, 42.2±2.2, 199.3±11.9, 167.7±15.9 0.05). CONCLUSION: Adiponectin can modulate endothelial inflammatory response through effecting the expressions of some inflammatory factors, and adiponectin has protective actions against the initiation and progression of atherosclerosis. 　　【Keywords】 adiponectin tumor necrosis factoralpha endothelium, vascular/cytology arteriosclerosis 　　【摘要】 目的： 探讨脂联素对肿瘤坏死因子（TNFα）引起的血管内皮细胞炎症反应的影响. 方法： 体外培养人脐静脉内皮细胞（HUVEC）. 随机分为对照组，TNFα刺激组和脂联素+TNFα刺激组. HUVEC与脂联素联合孵育一段时间后再给予TNFα刺激与单纯TNFα刺激做对照，分别采用化学法，