草珊瑚总黄酮提取及含量检测_医学论文.docVIP

草珊瑚总黄酮提取及含量检测_医学论文 草珊瑚总黄酮提取及含量检测_医学论文 【摘要】 目的 研究草珊瑚总黄酮的提取工艺。方法 以草珊瑚为原料，用乙醇为溶剂，利用超声波辅助提取和水浴法提取草珊瑚总黄酮，利用L9（33 ）正交实验方法优化总黄酮提取工艺，采用单因素实验，测定了乙醇浓度、料液比、提取时间、提取温度等单因素实验，考察总黄酮提取率与各因素的关系。根据单因素实验得出的显著条件进行正交设计，确定最佳工艺条件。结果 超声波法的最佳工艺条件为乙醇浓度50%、料液比1∶15、提取时间45 min，根据确定的最佳工艺条件，按实验中的测定方法测得提取的总黄酮含量为69.25 mg/g。水浴法的最佳工艺条件为乙醇浓度50%、料液比1∶20、提取温度80 ℃、提取时间2 h，按实验中的测定方法测得提取的总黄酮含量49.60 mg/g。结论 超声波提取不仅在时间上比水浴提取大大缩短，而且实验过程易于控制，黄酮提取率也比水浴提取提高28.38%。 【关键词】 草珊瑚；总黄酮；提取工艺；含量 ［Abstract］ Objective To optimize the extraction process of the flavonoids from sarcandra glaber.Methods Used sarcandra glaber as raw materials，took ethanol extraction methods and used ultrasonic water bath to extract the flavonoids，then used L9（33） orthogonal experiment to optimize the extraction process of flavonoids.One of the experiments is the single factor experiment.According to the given conditions and measured flavones，what we will do is to have single factor experiments about different concentrations of ethanol，feed ratio，extraction time and temperature.According to the remarkable conditions from single factor experiments，we will have an orthogonal design and then select the best conditions.Results The optimum conditions of ultrasonic methods are for the concentration of 50 percent ethanol，feed rate 1∶15，the extracted time 45 minutes，the flavones in 69.25 mg/g.The optimum conditions of water bath for ethanol methods are for concentration of 50 percent，feed rate 1∶20，the extracted temperature 80 ℃ and the flavones in 49.60 mg/g.Conclusion The time used in ultrasonic extraction is greatly shorter than that of water bath and the experiment is easier to control than that of water bath，and the extraction rate of flavonoids also raise 28.38% than that of water bath. ［Key words］ sarcandra glaber；flavonoids；extraction process；content 随着草珊瑚在医药、兽药、保健食品及保健用品领域中的应用越来越广泛，对其有效成分的提取分离显得越来越重要。黄酮类化合物是草珊瑚中含量最多、最主要的活性成分之一。目前，对草珊瑚总黄酮的提取分离工艺技术研究尚未见有详细报道，本学位论文拟对草珊瑚总黄酮提取分离工艺技术进行较系统的研究并筛选最佳新技术新工艺。采用正交实验方法考察沸水提取法及乙醇提取法对草珊瑚总黄酮提取效果的影响；采用中药高新工程技