葡萄膜炎患者外周血单个核细胞TLR9、TLR7 mRNA的表达及意义_医学论文.docVIP

葡萄膜炎患者外周血单个核细胞TLR9、TLR7 mRNA的表达及意义_医学论文 葡萄膜炎患者外周血单个核细胞TLR9、TLR7 mRNA的表达及意义_医学论文 作者：丁歆 付琳琳 苏稚辉 刘钢 方桂英 【摘要】 目的 观察葡萄膜炎患者外周血单个核细胞(PBMCs)Toll样受体9（TLR9）、Toll样受体7（TLR7）mRNA的表达。方法 收集42例葡萄膜炎患者（其中发病与感染相关者12例,与感染无关者30例)和正常对照组（30例)外周血,采用淋巴细胞分离液密度梯度离心法分离PBMCs,逆转录聚合酶链反应(RT-PCR)检测PBMCs TLR9、TLR7 mRNA表达的相对含量。结果 与感染相关葡萄膜炎患者PBMCs TLR9、TLR7 mRNA相对含量显著高于与感染无关葡萄膜炎患者和正常对照组(0.01)，与感染无关葡萄膜炎患者与正常对照组比较TLR9、TLR7 mRNA相对含量差异无统计学意义(Pgt0.05)。结论 TLR9、TLR97的表达在与感染相关葡萄膜炎患者中显著升高,提示感染因素在葡萄膜炎中的作用可能通过TLR9、TLR7介导。 【关键词】 葡萄膜炎外周血单个核细胞TLR9TLR7 Abstract: Objective To investigate the expression of TLR9 and TLR7 mRNA of peripheral blood mononuclear cells (PBMCs) in patients with uveitis. Methods Peripheral blood was collected from 42 uveitis patients (12 patients with or related to infection and 30 patients without) and 30 healthy individuals as controls. PBMCs were isolated by Ficoll-Hypaque gradient centrifugation. RT-PCR was adopted to determine the relative content of the expression of TLR9 mRNA and TLR7 mRNA of the PBMCs. Results The relative content of expression of TLR9 mRNA and TLR7 mRNA were significantly upregulated in the patients related to infection compared with that of patients unrelated and controls (0.01). There was no statistic significance for the differences in the content of expression of TLR9 mRNA and TLR7 mRNA between patients unrelated to infection and healthy controls (Pgt0.05). Conclusion The upregulated expression of TLR9 mRNA and TLR7 mRNA in uveitis patients associated with infection indicates that infective factors might be mediated by TLR9 and TLR7. Key words: uveitis peripheral blood mononuclear cells TLR9 TLR7 葡萄膜炎是一种T淋巴细胞介导的自身免疫性疾病，多发生于青壮年，易反复发作，治疗棘手，为致盲的主要原因之一。近年的研究表明，Th1型免疫反应在葡萄膜炎中起主导作用［1］。Toll样受体( Toll-like receptors, TLRs)是近年来备受关注的一种细胞表面信号传导跨膜受体，在识别细菌等病原微生物及介导的炎症反应信号通路中有重要作用，其表达异常与自身免疫性疾病关系密切［2］。TLR9 是TLRs家族中的重要一员，通过识别侵入机体的病毒和细菌中未甲基化CpG基序使免疫应答倾向于Th1型反应，TLR7识别病毒单链RNA，协同TLR9信号通路［3］。本研究初步观察了与感染相关及无关葡萄膜炎患者和正常对照组外周血单个核细胞（PBMCs）TLR9、TLR7 mRNA表达情况。 　　1 资料和方法 　　1.1 一般资料和