丛枝菌根真菌和有机土基质栽培对甜椒根际微生物分子多态性的影响_英文_.pdfVIP

Agricultural Science Technology, 2012, 13(5): 1021-1027 Copyright 訫 2012, Information Institute of HAAS. All rights reserved Soil and Fertilizer Effects of Arbuscular Mycorrhizal Fungus and Organic Substrate on the Molecular Diversity of Microbes in Sweet Pepper Rhizosphere under Protected Cultivation * Chaoxing HE , Linchuang WANG, Zhibin ZHANG Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China such as the low productivity, con- Abstract Denaturing gradient gel electrophoresis (DGGE) was used to study the ef- fects of different soil additives, i.e. control soil + Glomus mossea-2 (G.M), organic straints caused by the continuous substrate, organic substrate + G.M, on microbial molecular diversity in sweet pepper cropping, imbalance in soil microbiota, rhizosphere under protected cultivation. Results showed that following the inoculation soil secondary salinization, etc. During of G.M, the total number of bacterial species increased, with the appearance and the previous years, the application of disappearance of some bacterial species. Some bacteria, though detected in all treatments, showed increased cell/clone numbers with the addition of arbuscular myc- some organic cultivation techniques in orrhizal fungus G.M. The organic substrate favored the growth and propagation of protected vegetable production inte- some bacteria and was advantageous to the establishment of some dominant micro- grated with the straw and manure re- bial flora. Resulted from the similarity analyses of the DGGE profile,