甘薯抗逆相关基因IbERF3的克隆与表达分析.pdfVIP

华北农学报 ·2014 ,29(6):16-20 doi:10.7668/ hbnxb.2014.06.004 甘薯抗逆相关基因IbERF3 的克隆与表达分析 边小峰,谢一芝,郭小丁,贾赵东,马佩勇 (江苏省农业科学院粮食作物研究所,江苏 南京　 210014) 　 　 摘要:为了提高甘薯的抗逆能力,通过RACE 的方法克隆到一个含有AP2结构域的ERF家族基因,该家族基因在 植物逆境调控中起重要作用。 该基因cDNA全长1025 bp,编码区为669 bp,编码223个氨基酸,该基因在甘薯中未曾 报道,将其命名为IbERF3。 通过进化树分析发现,IbERF3在茄目类作物中具有一定的保守性。 通过定量PCR研究发 现,IbERF3在甘薯根、叶中都有表达,且在叶片中的表达量最高,同时研究发现,在干旱、盐胁迫处理后IbERF3 在根和 叶片中表达量都显著上升。 在用植物激素ABA处理后,IbERF3 的表达量逐渐上升并在24 h时达到最大。 因此,推测 IbERF3在甘薯抗逆途径中起重要作用。 关键词:甘薯;ERF;基因克隆;表达;非生物逆境 中图分类号:Q78;S531.03　 　 文献标识码:A　 　 文章编号:1000-7091(2014)06-0016-05 Cloning and Expression Profiles ofIbERF3,an Abiotic Stress Related Gene in Sweet Potato BIAN Xiao-feng,XIE Yi-zhi,GUO Xiao-ding,JIA Zhao-dong,MA Pei-yong (Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing　 210014) Abstract:In order to improve the stress resistance of sweet potato,we used RACE method to clone IbERF3 contained an AP2 domainwhich belongto ERF superfamily which playsan important role in stressresistance.Blast showed that IbERF3 wasanew genein sweetpotatothathadneverbeenreported.ThecDNAlength ofIbERF3 was 1205 bp,and contained an669 bp open reading frame encoding a223 amino acids.By homology analysis showed that IbERF3 was conserved in Solanaceae plants.Constitutive expression showed that IbERF3 express in root,leaf and stem,and theexpressionofIbERF3 wasthehighest inleaf.TheresultrevealthattheexpressionofIbERF3 was induced by drought,salt both in leaf and root by qRT-PCR.After treating with ABA,The expression of IbERF3 in- creased gradually and reached a maximum at 24 h.It suggests that IbERF3 plays significant roles in responses to abiotic stress in sweet potato. Key words:Sweet potato;ERF;Gene cloning;Expression;Abiotic stress