土壤细菌DNA提取及多样性分析的T-RFLP方法.pdfVIP

微生物学通报 JAN 20, 2008, 35(1): 131~136 Microbiology ©2008 by Institute of Microbiology, CAS tongbao@ 生物实验室 土壤细菌DNA 提取及多样性分析的 T-RFLP 方法 * 葛芸英 陈 松 胡 兰 凃 政 ( 100038) 摘 要: 获得高质量的土壤总 DNA 是土壤细菌生态学的关键步骤之一。实验通过综合应用两个 试剂盒(Soilmaster kit 和DNA IQTM 系统) 的优点进行土壤样品总DNA 的提取, 结果证明该方法是 一种快速、有效、灵敏、稳定的土壤DNA 提取方法。另外尝试将16S rDNA 序列和T-RFLP(Terminal restriction fragment length polymorphism)技术引入土壤细菌 DNA 群落多样性的研究中, 证明 T-RFLP 是一种有力的土壤细菌多样性分析工具。 关键词: 土壤细菌, 多样性分析, DNA 提取, T-RFLP 分析 A New Method of Soil Bacterial DNA Extraction and T-RFLP Analysis for Diversity * GE Yun-Ying CHEN Song HU Lan TU Zheng (Institute of Forensic Science, Ministry of Public Security, Beijing 100038) Abstract: Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study. A quick, efficient, sensitive and stably method of DNA extraction from soil were established by combining strong- points of two kits ( Soilmaster kit and DNA IQTM kit). In addition, the 16S rDNA gene and T-RFLP (Termi- nal restriction fragment length polymorphism) were used in the analysis of soil bacterial community diver- sity and the result show that T-RFLP is a powerful tool for bacterial community study. Keywords: Soil bacterial, Diversity analysis, DNA extraction, T-RFLP , , , , [1, 2] , , ,