阿魏酸钠对兔急性心肌梗死再灌注后无复流的影响_医学论文.docVIP

阿魏酸钠对兔急性心肌梗死再灌注后无复流的影响_医学论文 阿魏酸钠对兔急性心肌梗死再灌注后无复流的影响_医学论文 作者：丁玲岩 刘现亮 赵希军 王连文 韩兆东 曾璟 【摘要】 目的 探讨阿魏酸钠对兔急性心肌梗死（acute myocardial infarction，AMI )再灌注后无复流的作用及机制。方法 随机将24只成熟雄性新西兰大白兔分为假手术组、对照组、阿魏酸钠组3组，每组8只，分别建立AMI缺血再灌注模型，于缺血前5min、结扎后240 min，再灌注后120 min取静脉血。采用酶联免疫吸附法（ELISA）测定血清白介素（IL）；免疫组织化学法观察心肌组织P选择素（Ps）的变化；应用硫黄素染色评估无复流面积。结果 ①对照组和阿魏酸钠组AMI后240 min、再灌注后120 min的血清IL均显著高于假手术组(P均＜0.01)；阿魏酸钠组各时间点除AMI前血清IL均显著低于对照组(P均＜0.05)。②Ps免疫组化显示，对照组及阿魏酸钠组中，Ps在复流区及无复流区血管内皮细胞有强表达，明显强于正常区及假手术组（P均＜0.01）；阿魏酸钠组与对照组比较，Ps在复流区及无复流区表达均明显减弱（P均＜0.01）。③病理染色分析示，阿魏酸钠组结扎区心肌范围与对照组相比差异无统计学意义（P均＞0.05）；无复流区心肌范围与对照组相比显著降低(P均＜0.01 )。结论 阿魏酸钠能有效抑制炎症反应，防治AMI再灌注后无复流。 【关键词】 阿魏酸钠；炎症；白介素；P选择素；无复流 【Abstract】 Objective To evaluate effects of sodium ferulate on nond reperfusion.Methods Twentyfour mature male rabbits were randomly divided into shamoperated group，control group，sodium ferulate group with 8 in each group. The later groups were subjected to 240 minutes of left circumflex coronary artery (LCX) occlusion followed by 120 minutes of reperfusion. The serum levels of ILoperated group, IL6 in blood sample in control group and sodium ferulate group increased significantly at the end of 240 min of LCX occlusion and 120 min of reperfusion (all P＜0.01) In each time point but before AMI ILwas lower significantly than that in control group (all P＜0.05). ② In control and sodium ferulate groups, it was showed by IHC that Prmal myocardium and shamoperated groups（all P＜0.01）In sodium ferulate group, its expression in reflow and no（all P＜0.01 ）.③Between sodium ferulate group and control group, there was no significant diference in coronary ligation area (P＞0.05)The area of no＜0.01 ).Conclusion Sodium ferulate is effective in antiinflammation and preventing myocardial noflow in rabbit with acute myocardial infarction and reperfusion. 【Key words】 sodium ferulate,inflammation,interleukin 无复流是指急性心肌梗死（AMI）冠脉再通后，心肌组织再灌注不完全，甚至出现无再灌注。中性粒细胞、纤维蛋白血栓、血小板的聚集，内皮细胞肿胀等因素造成微循环障碍是无复流现象的重要标志［1］。炎症在缺血性心脏病的发生与发展中起着关键作用［2］。因此，近年来，从免疫学的角度来研究心肌缺血再