非何杰金淋巴瘤微血管密度及其血清bFGF水平_医学论文.docVIP

非何杰金淋巴瘤微血管密度及其血清bFGF水平_医学论文 非何杰金淋巴瘤微血管密度及其血清bFGF水平_医学论文 【关键词】 碱性成纤维细胞生长因子 　　Tissue microvessel density and levels of serum basic fibroblast growth factor in patients with nonHodgkin’s lymphoma 　　【Abstract】 AIM: To explore the levels of serum basic fibroblast growth factor (bFGF) and the tissue microvessel density (MVD) in nonHodgkin’s lymphoma (NHL) patients with different clinical and pathological features. METHODS: In 32 NHL patients (13 newly diagnosed and 19 in remission after chemotherapy) and 10 healthy controls, ELISA was used to detect their serum level of bFGF and FⅧ RAG was used as the marker of endothelial cells to detect MVD in 20 cases of the 32 NHL patients and 10 cases of benign lymphadenopathy. The statistic t test was used to analyze the difference among different groups. RESULTS: The mean concentrations of serum bFGF in the untreated patients and patients in remission after chemotherapy were（7.6±1.2) ng#12539L-1 and （4.1±1.0）ng#12539L-1, respectively, which were higher than that ［（1.7±1.4）pg#12539L-1］ in healthy controls (0.05). The serum bFGF level in Tcell type NHL was significantly higher than that in Bcell type ［(10.7±1.4) ng#12539L-1 vs （2.7±1.0）ng#12539L-1, 0.05］. The mean MVD of the 20 NHL cases (39±18) was obviously higher than that in benign lymphadenopathy group (13±5, 0.05) and the mean MVD in Tcell type NHL was significantly higher than that in Bcell type（47±15 vs 27±17, 0.05）. CONCLUSION: The NHL patients, especially those with Tcell type NHL, have significantly higher serum bFGF levels and tissue MVD than controls. The data presented here strongly suggest that the bFGFmediated angiogenesis may closely be correlated with the pathogenesis and pathologic characteristics in NHL. 　　【Keywords】 basic fibroblast growth factor nonHodgkin’s lymphoma ELISA microvessel density immunohistochemistry 　　【摘要】 目的: 分析不同临床特征和病理分型的非何杰金淋巴瘤(NHL)患者的血清碱性成纤维细胞生长因子(bFGF)水平及瘤性组织微血管密度（MVD）的差异. 方法: 收集32（初发未经化疗13，化疗后缓解19）例NHL患者及10例健康对照者血清，采用夹心ELISA法测定其bFGF水平；采用vWF抗