体外构建与体内构建组织工程的比较研究.pdfVIP

·857 ·实 验 研 究 · 体外构建与体内构建组织工程的比较研究 戚超 杨志明 黄富国 秦廷武 罗静聪 蔡谈 【摘要】 目的 比较骨髓基质干细胞(MSCS)与生物衍生骨体内、体外复合构建的组织工程骨 修复山羊胫骨大段缺损能力，以探讨修复缺损最佳途径。方法 制备山羊胫骨中段20mm的骨一骨 膜缺损，分别植入体内、体外构建的组织工程骨、自体骨及单纯生物衍生骨，分别行组织学、X线观 察、骨密度测定和生物力学测试。结果 体外构建组材料降解较快，成骨迅速，16周时已可见髓腔 再通;体内构建组以上各变化较前者慢2-4周;而单纯生物衍生骨对照组材料降解、新骨形成均较 慢;;6周时，各组弯曲应力值差异无显著性(P0.05),12,16周时体外构建组高于体内构建组(P 0.05)，体内构建组高于单纯生物衍生骨对照组(P0.05).结论 体内、体外构建的组织工程骨成 骨迅速、量大，排斥反应小;但是体内构建方式成骨较体外构建慢。 【关健词] 组织工程骨;体内构建;体外构建;骨髓基质干细胞;生物衍生骨 Comparisonstudyoftissueengineeringboneconstructedinvivoandinvitro QIChao,YANGZhi- ming,HUANGFu-guo,etal.DivisionofStemCellandTissueEngineering,KeyLaboratoryofBio- therapyofHumanDiseasesofMinistryofEducation，SichuanUniversity,Chengdu610044,China IAbsract)ObjectiveToinvestigatetheabilityofripairinggoattibiadefectwiththetissueengi- neeringboneconstructedbymarrowstromalcells(MSCs)andbio-derivedboneinvitroandinvivo,and findthebestwaytorepairlargebonedefect.Methods The20mmtibiadefectofgoatwasmadeand fixedwithplate.Theyweredividedinto4groupsandfilledwiththetissueengineeringboneconstructed invitroandinvivo,autograft-boneandbio-derivedbonedifferently.Theywereassessedbyhistopatholo- gical,X-ray,bonemineraldensityexaminationsandbiomechanicalexamination.Results Thegroupof thetissueengineeringboneconstructedinvivodegradedquickly,sowasthenewboneformation.Thede- fectsofthisgroupwereperfectlyrepairedat16weeks,itwasfasterthanthegroupofwhichconstructed invivoabout2-4weeks.Butthenewboneformationofgroupofbio-derivedbonewasslowly.Bybiome- chanicalexamination,fourgroupshadntstatisticallysignificantdifferenceat6weeks(P0.05).At 12,16weeks,theeffectsofbiomechanicshadst