快速提取活性蛋白的方法.pdfVIP

产品说明书产品说明书 / Specification Sheet 产品说明书产品说明书 一步法细菌活性蛋白提取试剂一步法细菌活性蛋白提取试剂 一步法细菌活性蛋白提取试剂一步法细菌活性蛋白提取试剂 BSP023  :  ,  !#$%()*+,-./01234567809:!!;=%?@ABCD $0EFGH7I)*JKLMN(1=$%@OPQRSTU!;9VW X)*12J!:Y N(1=$%/Z[\]^%?_(`aPbcQRSTU!;9VW X)*deLysozyme / DNase I 12bfGgKc60kDa 1=$%/%\]^hijkZ[`a(012=$%12lm, c nopqjk%rstuvPull Down EMSAwof6 (Western blot, ELISA, RIA)Sxyz1 20 2{, |z }~}~: }~}~ ()* 25ml Lysozyme: 0.1 ml DNase I: 0.1 ml PMSF 300ul DTT 30ul €‚ƒ„…€‚ƒ„…: €‚ƒ„…€‚ƒ„… 2-8 , Lysozyme, DNase I DTT PMSF -20 , †.‡ˆ‰[Š‹Œ()* Ž ŽŽr‘z ’“’“: ’“’“ 1. 20 12” f•–—˜™!1=$%0(z 2. X)*12š›xœ1=$%(/%\]^hijkZ[z 3. , , Pull Down EMSA (012=$%12lm,c nopqjk%rtzuv z Sxyz 4. (0%r125678FG/7I)*žŒŸ Y¡¢£¤OP0¥0%r0¦§Ÿ Yz 5. 12¨©OP¨ª«¬K(0^0gª­¦§c®¯%0°±²³z ´µ¶·´µ¶· ´µ¶·´µ¶· 1. 5,000 × g 10 F¸ f•[¹!!;jkz º»¼½¾OP12”¿ÀÁ0()*NÂÃ2 µl of Lysozyme / 2 µl of DNase Iz 2. 100mg 0.5ml ( 1mL 1ul DTT 10ul PMSF ¨©¿ 0Ä®^NÂà 0()*{,Å¿ ()*Âà Æ, ÇÈÉÊËÌ{ÍΘÏÐ0ÑYz 3. 280rpm 20 ŒÒÓԔÕÖ×Ø Ù.ÚÛ f•ÜÝz : . Lysozyme DNase I, 37 º»xÞOP›ß12àá!0@šâ-ãäå½¾()*NæçÂÃè / é” ÓÔ2êë™!!;ì/íî0ïðz 4. 15,000 × g 5 F¸ f•[¹ñò£!12=$óf@=0!;Vô/1õö÷0\]^0jkøò f6žZ[,z º»¼®Êù2-4 |Œú|0ñ¦û12b1=$%0(`a½¾üâ”!;:‹½¾ Dýþÿéë)*N§g–ÂDNase I2ï