安全转基因技术研究进展.pdfVIP

HEREDITAS (Beijing) 2011 5 , 33(5): 437―442 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2011.00437 安全转基因技术研究进展 1 1 1 2 3 张茜 , 张金凤 , 付文锋 , 张鸿景 , 袁文军 1. , , , , 100083; 2. , 050061; 3. , 068450 转基因生物安全性问题已引起了公众的普遍担忧, 严重制约了转基因技术成果的推广应用。近年来, 研 究者探索了不同的技术策略来解决转基因生物的安全性问题, 安全转基因技术成为当前转基因研究的热点之 一。文章介绍并评价了几种主要的安全转基因技术, 包括无选择性标记基因技术、安全标记基因技术、叶绿体 转化技术、终止子技术、雄性不育技术、外源基因删除技术。其中, 外源基因删除技术为实现转基因生物的安 全应用展示了诱人的前景。最后, 对探索新的安全转基因技术策略保障转基因生物安全提出了建议。 转基因生物; 生物安全性; 安全转基因技术; 外源基因删除技术 Advances on transgene containment technologies 1 1 1 2 3 ZHANG Qian , ZHANG Jin-Feng , FU Wen-Feng , ZHANG Hong-Jing , YUAN Wen-Jun 1. National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education , the Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, China; 2. Hebei Academy of Forestry Science, Shijiazhuang 050061, China; 3. Forest Bureau of Weichang Mancu and Mogolia Autonomy County, Weichang 068450, China Abstract: The biosecurity of transgenic organism has been widely concerned and extremely restricted its application. Recently, many technological strategies have been developed to ensure its biosecurity. Thus, transgene containment tech- nologies have become one of the hotspots in current transgenic research. In this paper, several trans