去甲肾上腺素对胰腺癌细胞株MiaPaCa2侵袭能力的影响_临床医学论文.docVIP

去甲肾上腺素对胰腺癌细胞株MiaPaCa侵袭能力的影响_临床医学论文 去甲肾上腺素对胰腺癌细胞株MiaPaCa侵袭能力的影响_临床医学论文 作者：郭坤，沙焕臣，马清涌，王连才，胡恒通 【摘要】 目的 探讨经典神经递质去甲肾上腺素(norepinephrine, NE)对人胰腺癌细胞株MiaPaCa侵袭能力的影响及其作用机制。方法 取人胰腺癌细胞株MiaPaCa为研究对象，实验分为空白对照组、0.1μmol/L NE干预组、1μmol/L NE干预组和10μmol/L NE干预组。干预48h后，采用Transwell侵袭实验检测各组中MiaPaCa细胞侵袭能力的变化;RT法检测细胞中MMP、MMP和VEGF mRNA的表达;免疫细胞化学法检测细胞中MMP、MMP和VEGF蛋白的表达。结果 1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa细胞的侵袭能力均高于空白对照组(0.01);1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa细胞中MMP、MMP和VEGF mRNA和蛋白表达水平均高于空白对照组(0.05)。结论 NE可以通过上调MMP、MMP和VEGF的表达从而促进胰腺癌细胞发生远处侵袭转移。 【关键词】 胰腺癌;细胞侵袭;神经递质;去甲肾上腺素 　　ABSTRACT: Objective To investigate the effect of norepinephrine (NE) on the invasiveness of pancreatic cancer cell line MiaPaCa in the research. The cells were randomly divided into control group, 0.1μmol/L NE intervention group, 1μmol/L NE intervention group and 10μmol/L NE intervention group. After intervention for 48h, Transwell invasiveness test was given to examine the changes of invasive ability of MiaPaCa2. The expressions of MMP2 mRNA, MMP9 mRNA and VEGF mRNA were measured by semiquantitative RTPCR; the levels of MMP2, MMP9 and VEGF proteins were measured by immunocytochemical method. Results The invasive ability of MiaPaCa2 in 1μmol/L NE intervention group and 10μmol/L NE intervention group was significantly higher than that in control group (0.01). The levels of MMP2, MMP9 and VEGF mRNA and protein expression of MiaPaCa2 in 1μmol/L NE intervention group and 10μmol/L NE intervention group were higher than those in control group (0.05). Conclusion NE can promote the invasiveness of MiaPaCa2 through upregulating the expressions of MMP2, MMP9 and VEGF. 　　KEY WORDS: pancreatic cancer; cell invasiveness; neurotransmitter; norepinephrine 　胰腺癌具有侵袭力强、早期即发生转移的特点，5年生存率不足5%，其主要原因是患者在就诊时肿瘤就已经发生了远处转移。肿瘤细胞侵袭能力增强是肿瘤远处转移的先决条件。所以，研究胰腺癌细胞侵袭能力的影响因素对指导临床诊治有重要意义。近年来，肿瘤细胞微环境中的信号物质对肿瘤细胞侵袭能力的影响成为研究的热点，这些信号物质包括趋化因子和神经递质两类[1]。本研究旨在通过观察胰腺癌细胞株MiaPaCa在不同浓度经典神经递质去甲肾上腺素(norepinephrine, NE)的干预下侵袭能力的变化，以及细胞中MMP、MMP、VEGF mRNA和蛋白表达