双后肢大鼠增龄制备颈椎间盘退变动物模型_临床医学论文.docVIP

双后肢大鼠增龄制备颈椎间盘退变动物模型_临床医学论文 双后肢大鼠增龄制备颈椎间盘退变动物模型_临床医学论文 王卫明，金大地，瞿东滨 【摘要】 目的 通过截除幼年大鼠双前肢建立颈椎间盘退变的动物模型。方法 健康4周龄雄性SD大鼠76只，随机分为两组，手术组40只，对照组36只。手术组动物麻醉后于双上肢根部截除肢体，分别于术后3、6、9、12个月随机分为4组，每组10只处死大鼠后摄颈椎正侧位X线片，并切取椎体标本制备C2～C3至T2～T3的中矢状面组织学切片，分别进行HE、番红O染色行椎间盘组织学观察。对照组动物分别于实验开始后4、8、12、16个月分为4组进行与实验组相同的处理。结果 截除双前肢后，双后肢大鼠全部存活，实验组大鼠术后9、12个月均出现颈椎间盘退变的X线征象，光镜检查证实其椎间盘均发生了严重的退变，脊索性髓核完全为纤维软骨性髓核取代，软骨终板缺损或消失，基质中胶原纤维排列紊乱，椎间盘突出并骨化。而正常大鼠的椎间盘仅发生轻度退变。结论 本造模方法不损伤动物靶器官正常的解剖结构，成功率高、重复性好，符合人类颈椎间盘退变规律。 【关键词】 疾病模型；动物；颈椎间盘；X线摄片；组织学 【Abstract】 Objective To develop a scientific and reproducible degenerative disc animal model for the study on the cervical disc.Methods The bipedal rats model was developed in 40 infancy SD rats by forelimb amputation.Thirty-six normal rats in the same age served as the control.When the rats in the experimental group were 3，6，9 and 12 months (named E3，E6，E9 and E12 group respectively) and in the control group were 4，8，12 and 16 months (named C4，C8，C12 and C16 group respectively) postoperatively，the vertebral columns from L2～3 to T2～3 were removed and observed under radiographic and histological examination after the rats were sacrificed.Results After forelimb amputation，all the bipedal rats survived.Overload on the cervical spine elicited by this surgical intervention accelerated the process of cervical intervertebral disc degeneration.Radiographic studies of the vertebral columns of all the animals in the E9 and E12 groups revealed evidence of degeneration characterized by disc-space narrowing，anterior osteophyte formation and disorder alignment.Light microscopy revealed that the degeneration of the nucleus pulposus and cartilage endplate were severe and the collagen fibers in the matrix were irregular in bipedal rats’discs.Cervical disc herniation was found in some of the bipedal rats.On the other hand，mild degeneration signs were found in the control group.Conclusion The availability of this experimental model should be valuable for comprehensive understanding of the