吡咯烷二硫代氨基甲酸酯对2型糖尿病大鼠肝脏的保护作用_临床医学论文.docVIP

吡咯烷二硫代氨基甲酸酯对2型糖尿病大鼠肝脏的保护作用_临床医学论文 吡咯烷二硫代氨基甲酸酯对2型糖尿病大鼠肝脏的保护作用_临床医学论文 作者：张利众 赵瑞景3 刘建坤 高书涛 朱铁年 【摘要】 目的 探讨吡咯烷二硫代氨基甲酸酯(pyrrolidine dithiocarbamate，PDTC)对2型糖尿病大鼠肝脏的保护作用及机制。方法 雄性Wistar大鼠，随机分为两组，正常对照组和高脂饮食组。喂养8 w后，高脂组腹腔注射小剂量链脲佐菌素(STZ，27 mg/kg)制造2型糖尿病大鼠模型，造模成功后随机分为两组：糖尿病模型组和PDTC治疗组。治疗1 w后断头处死大鼠，测定血脂，HE染色观察大鼠肝脏形态学变化，RT检测iNOS mRNA 的表达。结果 糖尿病模型组较正常组血脂明显升高(0.01)，PDTC治疗组与模型组相比无明显差别(Pgt;0.05);糖尿病模型组与正常组相比较肝脏明显脂肪变性，肝细胞损害，PDTC治疗组肝细胞脂肪变性及损害明显减轻。糖尿病模型组iNOS mRNA 的表达较正常组明显升高(0.01)，经PDTC治疗后表达较糖尿病模型组降低(0.01)。结论 PDTC可以减少iNOS mRNA 的表达，减少肝细胞脂肪变性及损伤。 【关键词】 吡咯烷二硫代氨基甲酸酯;糖尿病;脂肪肝;诱生型一氧化氮合酶 　　【Abstract】 Objective To determine the protective effects of pyrrolidine dithiocarbamate on hepatic and its mechanism in diabetic rats.Methods Male Wistar rats were randomly divided into normaleight weeks of feeding, highfat group was given lowdose streptozotocin (STZ, 27 mg/kg ) intraperitoneal injection. After type 2 diabetes mellitus(T2DM) rat models were established, T2DM rats were randomly divided into diabetic model and diabeticPDTCtreated groups. After the rats were treated for a week, blood lipid levels were determined and livers were staining. The levels of iNOS mRNA were detected by RTPCR. Results Blood lipid level of diabetic model group was significantly higher than that of normalcontrol group(0.01), and no difference existed between diabeticPDTCtreated group and diabetic model group(Pgt;0.05). PDTC significantly reduced liver cell steatosis and damage compared with those of diabetic model group. The expression of iNOS mRNA was significantly higher in diabetic model group(0.01), and the expression in diabeticPDTCtreated group was significantly decreased than that of the diabetic model group(0.01). Conclusions PDTC can reduce the expression of iNOS mRNA and liver steatosis and injury. 　　【Key words】 Pyrrolidine dithiocarbamate;Diabetes;Fatty liver;iNOS 　　 随着糖尿病患病率的增加，出现糖尿病并发症的患者逐年增多。对于糖尿病并发症人们关注最多的是血管及神经系统病变，而对于糖尿病合并肝脏病变研究较少，有研究表明糖尿病患者中合并肝脏病变高达50%以上〔1〕。本文从氧化损伤角度探讨吡咯烷二硫代氨基甲酸酯(pyrrolidine di