安蓝风湿药液急性毒性实验和镇痛作用的研究_临床医学论文.docVIP

安蓝风湿药液急性毒性实验和镇痛作用的研究_临床医学论文 安蓝风湿药液急性毒性实验和镇痛作用的研究_临床医学论文 作者：曾泽民,李雪艳,肖祥书； 潘善庆 , 陈子渊 【摘要】 目的 （1）观察小鼠口服安蓝风湿药液所产生的急性毒性反应及死亡情况，为临床试验安全性提供资料。（2）通过对小鼠镇痛实验观察，对临床用药提供依据。方法 （1）经10只小鼠预试，ig测不出LD50，故进行最大给药量测定。（2）对醋酸致痛小鼠扭体次数的影响；对热板法致痛小鼠痛阈的影响。结果 （1）小鼠ig安蓝风湿药液每日最大给药量为180g生药/kg相当于临床60kg成人一日量的400倍；（2）安蓝风湿药液高、中、低三个剂量组和阿司匹林阳性对照组均明显抑制醋酸致痛小鼠扭体次数的增加，均能明显提高给药后30、60、90、120min热刺激小鼠的痛阈值，其中安蓝风湿药液高、中剂量组和阿司匹林阳性组均能明显提高醋酸致痛小鼠扭体的替伏期。结论 （1）安蓝风湿药液毒性小，在临床推荐的用药剂量下使用是安全的；（2）安蓝风湿药液具有明显的镇痛作用。 【关键词】 安蓝风湿药液；急性毒性试验；镇痛实验 Research on the acute toxic test and the analgesia effect of the Anlar fengshi solution 【Abstract】 Objective （1）Observation mice with oral Anlan fengshi solution produces acute toxic response and death situation, provides the material for the clinical test security.（2） Through mice analgesia experiment observation, provides the basis with the medicine. Method （1） After 10 mice preparations, ig cannot measure LD50,therefore carries on survey quota for most greatly gives the doses.（2） To acetic acid causes the ache mice sways from side to side the body number of times;Of Reban method causes the ache mouse ache scope influence. Results （1） Mice ig Anlan fengshi solution most greatly gives the dose every day is 180g drugs/kg,equal to clinical 60 kilograms grown-up person every day amount used 400 times;（2） Anlan fengshi solution high, middle,smaller three amount used with aspirin tablets positive comparison group all obvious suppression the acetic acid causes the ache of the mouse sways from side to side the bodily number of times the increase,all can obviously enhance for drugs after 30th, 60, 90, 120min.The heat stimulates mouse’s pain threshold value，among Anlan fengshi solution high, middle amount used with aspirin tablets positive comparison group all can obviously enhance the acetic acid causes the ache mouse sways from side to side of the body for bends down the time. Conclusion （1） Anlan fengshi solution toxicity is small,uses under the medicine dosage in the clinical recommendation the