宣肺健脾中药对哮喘大鼠支气管-肺组织病理学和转化生长因子-β1的影响_临床医学论文.docVIP

宣肺健脾中药对哮喘大鼠支气管-肺组织病理学和转化生长因子-β1的影响_临床医学论文 宣肺健脾中药对哮喘大鼠支气管-肺组织病理学和转化生长因子-β1的影响_临床医学论文 作者：马佐英,何山,袁卫玲,郭茂娟,王学岭 【摘要】 目的 观察宣肺健脾中药哮逐平对支气管哮喘大鼠的治疗作用,探讨其可能的作用机制。方法 SPF级雄性Wistar大鼠32只,随机分为正常对照组(A组)、哮喘模型组(B组)、地塞米松组(C组)、哮逐平组(D组),每组8只。除A组外,其余组动物建立哮喘模型。HE染色,观察支气管-肺组织病理学改变,以医学图像分析软件测量支气管基底膜周径(Pbm)、支气管壁厚度(Wat)和平滑肌厚度(Wam)。免疫组化PV二步法染色,应用上述图像分析软件检测支气管-肺组织转化生长因子-β1(TGF-β1)的积分光密度(IOD)。结果 与A组相比,B组Wat、Wam和支气管-肺组织TGF-β1含量明显增加,差异有统计学意义(P＜0.01)。经药物干预后,C、D组Wat、Wam减少及支气管-肺组织TGF-β1含量显著降低(P＜0.05)。D组Wat、Wam和TGF-β1减少尤为明显,与B组相比,差异有统计学意义(P＜0.05),其中Wat、Wam与A组相比,差异无统计学意义(P＞0.05)。与C组相比,D组TGF-β1含量明显下降,差异有统计学意义(P＜0.01)。结论 宣肺健脾中药可能通过抑制哮喘大鼠支气管-肺组织TGF-β1的过度表达而影响平滑肌增殖、减轻气道壁增厚,干预气道重塑。 【关键词】 宣肺健脾中药；哮逐平；哮喘；气道重塑；转化生长因子-β1；大鼠 　　Abstract：Objective To observe the effect of bronchial asthma in rats treated by Chuanzhuping, a prescription which has the function of dispersing lung Qi and invigorating spleen Qi, and explore its possible mechanism. Method Thirty-two male Wistar rats of SPF level were divided into four groups randomly：control group (A), asthma group (B), Dexamethasone group (C) and Xiaozhuping group (D). Except group A, the others were established animal model of asthma. After drawing materials, hematoxylin and eosin (HE) staining were performed. The pathological changes in bronchopulmonary tissue of rats were detected. The basementmembrane perimeter (Pbm), the airwaywall area (Wat) and the smooth musclewall area (Wam) were measured and calculated by using image analysis system. The concentration of transforming growth factor-β1 (TGF-β1) in bronchopulmonary tissue was observed by immunohistochemical method and the integral optical density (IOD values) of it was measured. Results In bronchopulmonary tissue of group B, Wat, Wam and the concentration of TGF-β1 increased, and were significantly different from that in group A (P＜0.01). In group C and D, Wat, Wam and the concentration of TGF-β1 decreased, and the concentration of TGF-β1 was significantly different from that in group B (P＜0.05). In group D, Wat a