盐胁迫下骆驼刺组织中钠离子和氯离子测定.docVIP

目 录 摘要································································1 关键词······························································1 前言································································2 1.材料与方法·······················································3 1.1 试验材料·······················································3 1.1.1 植物样品···················································3 1.1.2 试剂·······················································3 1.1.3 主要仪器····················································3 1.2 试验方法·······················································3 1.2.1 试验材料的准备·············································3 1.2.2植物样品的前处理············································4 1.2.3测定方法····················································4 1.2.4 标准曲线的绘制·············································5 2 .结果与分析······················································5 2.1 样品液测定·····················································5 2.1.1 骆驼刺中氯离子的测定········································5 2.1.2 骆驼刺中钠离子的测定········································6 2.2 标准曲线的分析·················································7 2.3 实验数据的重复性分析···········································7 2.4 盐胁迫对骆驼刺体内Na+和Cl_含量影响·······························7 4结论·····························································9 谢 辞····························································10 参考文献 ·························································11 盐胁迫下骆驼刺组织中钠离子和氯离子的测定 杜曼·加勒卡森 摘要：针对在西北绿化中的使用, 本论文用0溶液分别对种子进行胁迫处理,20001x的条件下无菌培养0、5、10、15天的骆驼刺用碳酸钠－碳酸氢钠淋洗液Na+和Cl-的含量的变化与盐胁迫的关系。结果表明:随着NaCl胁迫时间和浓度的增加,骆驼刺中Na+、Cl-的含量增加，这也正说明了具有一定的耐盐能力。当盐浓度达到一定程度时，Na+开始下降表明随着盐分浓度的升高，对细胞膜产生伤害种子发芽、幼苗生长（）能忍耐的盐液（）胁迫。(Alhagi sparsifolia)为豆科(Leguminosae)蝶形花亚科( Pap ilionatae)骆驼刺(Alhagi Gagneb. )的半灌木，该植物广泛分布在亚欧大陆的干旱或半干旱地区及非洲部分地区。在我国主要分布在大西北的宁夏，甘肃，新疆及内蒙古等地区。新疆的分布面积达1.73×106 hm2，占新疆草地面积的3.03％。集中分布在巴州地区，阿克苏地区，哈密地区，喀什地区和吐鲁番等地区。新疆有骆驼刺属植物3个种，即疏叶骆驼刺，小叶骆驼刺和骆驼刺等[1,2]。它生境条件属于低平盐渍化沙地、具有很强的抗旱，抗霜冻，耐盐碱能力。不但是改良土壤质地的最佳植被而且当地良好的饲草饲料