25血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖.docVIP

血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖、 凋亡和血管形成的调节 富奇志1，齐志国1,朱晓峰2,谢 鹏3 (400016 重庆，重庆医科大学：第一附属医院神经内科1，神经科学中心3；154002 黑龙江 佳木斯，佳木斯大学神经科学中心2) ［提要］ 目的 研究血管内皮祖细胞（endothelial progenitor cells, EPCs）与神经干细胞（neuron stem cells, NSCs）构建的复合移植体对移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖、凋亡和血管重建的影响。方法 线栓法建大鼠脑缺血再灌注模型；血管内皮祖细胞、层粘连蛋白和神经干细胞构建的移植复合体移入模型鼠脑缺血半暗带；免疫组化观察Brdu标记的移入脑内神经干细胞和缺血半暗带血管新生，免疫荧光双标观察神经干细胞的凋亡情况。结果 移植后各时间点Brdu标记的神经干细胞数NSCs+EPCs组明显高于NSCs组（P0.05）；NSCs+EPCs组在各时间点的凋亡率明显低于NSCs组（P0.05）；各时间点NSCs+EPCs组血管新生数量明显多于EPCs组、缺血对照组和NSCs组（P0.05）。结论 血管内皮祖细胞可以促进移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖，减少其凋亡；2种细胞共移植可以促进缺血半暗带的血管新生。 ［关键词］ 神经干细胞；毛细血管内皮祖细胞；缺血再灌注 ［中图法分类号］ R322.81;R329.28;R743.31 ［文献标识码］ A Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural stem cells and angiogenesis of MCAO/R rats Fu Qizhi1, Qi Zhiguo1, Zhu Xiaofeng2, Xie Peng3 (1Department of Neurology, First Affiliated Hospital, 3Neuroscience Center, Chongqing Medical University, Chongqing, 400016; 2Neuroscience Center, Jiamusi University, Jiamusi, Heilongjiang Province, 154002, China) ［Abstract］ Objective To investigate the effect of endothelial progenitor cells (EPCs) which complexed with neuron stem cells (NSCs) after transplanted into ischemia and reperfusion (I/R) rat model on the proliferation and apoptosis of the NSCs and vasal construction. Methods Totally 150 SD adult male rats were randomly and equally divided into 5 groups, sham operation group, I/R group, I/R+NSCs group, I/R+EPCs group, and I/R+NSCs+EPCs group. The rats were further divided into 5 subgroups according to the time points of 3, 7, 14, 30 and 60 d after transplantation. I/R rat model was established by reversiblyligating middle cerebral artery occlusion. NSCs were derived from the hippocampus of SD rats born in 24 h and identified with Nestin staining. EPCs were obtained from the artery blood of SD rats and verified with immunocytochemical stainings of CD31, CD34 and KDR after culture. The complex of NSCs