上海地区猪群中H1N1 甲型流感抗体检测.pdfVIP

中国动物传染病学报 2011,19(2): 62-65 Chinese Journal of Animal Infectious Diseases ·简报· 上海地区猪群中 H1N1 甲型流感抗体检测 李凯航，葛菲菲，薛　霞，鞠龚讷，周锦萍 201103 ( 上海市动物疫病预防控制中心，上海 ) 对 年 甲型流感流行前后的上海地区养殖场户 410 份猪血清样品，分别采用血凝抑制试验（ 摘　要： 2009 H1N1 hemagglutination ， ）和酶联免疫吸附试验（ ， ）进行检测 H1N1 甲型流感病毒和猪流感 inhibition HI enzyme-linked immunosorbent assay ELISA 病毒（Swine influenza virus ，SIV）。检测结果表明，除 2007 年外，2008~ 2010 年猪血清中均存在不同水平的 HI 抗体，阳 性率呈显著上升趋势，且抗体水平与猪群饲养周期及饲养密度正相关，而与猪流感病毒的流行无相关性。 甲型流感病毒； 亚型猪流感；血凝抑制试验； 酶联免疫吸附试验 关键词：H1N1 H1N1 S858.282.659.5 B 1674-6422(2011)02-0062-04 中图分类号： 　　　　　　　　　文献标志码： 　　　　　　　　　文章编号： DETECTING OF ANTIBODIES AGAINST INFLUENZA A VIRUS H1N1 IN SWINE HERDS IN SHANGHAI LI Kai-hang, GE Fei-fei, XUE Xia, JU Gong-ne, ZHOU Jin-ping (Shanghai Centers for Animal Disease Control and Prevention, Shanghai 201103, China) Abstract: Detected antibodies of 410 sera derived from different swine herds in shanghai during influenza A virus H1N1 prevalence against influenza A virus H1N1 subtype and SIV by HI test and ELISA, respectively. The results showed that, except in 2007, antibodies against the new influenza A virus H1N1 subtype were at different levels in 2008-2010, and the positive rates showed a significant upward trend; the antibody levels were associated with herd breeding period and density, not were correlated to the SIV prevalence. Key words: Influenza A virus H1N1 subtype; H1N1 subtype swine influenza; hemagglutination inhibition test; enzyme-linked immunosorbent assay 2009 年 3 月，一种新型 H1N