Growing Cells in Culture.ppt

What to do with growing cells If they are at least 70-80% confluent Subculture them Also called passing or splitting Remove media, remove cells, resuspend and transfer some to a new plate If they are not very confluent Lift and replace onto same plate Culture more than 4 days old for our cells Remove old media, lift cells from plate and resuspend in fresh media on same plate “Feed” them Culture less than 4 days old Remove old media and replace with fresh, warm media Brief subculturing preview Remove media, lift cells from plate Resuspend cells in fresh media Count cells and determine viabili