p14ARF、mdm2、p53在鼻咽癌中的表达及其临床意义.docVIP

p14ARF、mdm2、p53在鼻咽癌中的表达及其临床意义 　　作者：杨治花,折虹,丁喆　　作者单位：宁夏医科大学附属医院，宁夏 银川 　　【摘要】 目的 研究p14ARF、mdm2、p53在鼻咽癌组织中的表达与临床分期、淋巴结转移、预后等因素的关系。方法 应用免疫化法检测58例鼻咽癌组织和20例慢性鼻咽炎症组织中p14ARF、mdm2、p53表达情况。结果 (1)p14ARF、mdm2、p53在鼻咽癌组织和慢性鼻咽炎症组织中的阳性表达率分别为31.0%、41.4%、65.5%和100%、0、0，差异有统计学意义(P0.05);(2)Spearman相关分析表明，鼻咽癌组织中p14ARF、mdm2、p53三者表达之间均无相关性;(3)p14ARF、mdm2、p53表达与鼻咽癌淋巴结转移、分期、性别、年龄无关;(4)Kaplan-Meier分析方法显示鼻咽癌组织中mdm2表达与生存时间相关(P0.05);p14ARF、p53表达与生存时间无关。结论 p14ARF、mdm2、p53可能参与了鼻咽癌的发生;mdm2表达可作为预测鼻咽癌放疗预后的一项参考指标。 　　【关键词】 鼻咽肿瘤;p14ARF;mdm2;p53;免疫组织化学 　　The expression and clinical significance of p14ARF, mdm2, p53 protein in nasopharyngeal carcinoma　YANG Zhi-hua，ZHE Hong，DING Zhe　(The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Chian) 　[Abstract] Objective To investigate the role of p14ARF, mdm2 and p53 protein in the process of carcinogenesis of nasopharyngeal carcinoma; analyze the relationship among the expression and clinical stage, lymph node metastasis, prognosis, et al. Methods The expression of p14ARF, mdm2 and p53 protein were detected by immunohistochemical method in 78 paraffin-embedded tissues, including nasopharyngeal carcinoma (NPC) (n=58), chronic inflammation of nasopharyngeal epithelium (CINE) (n=20). Results (1) p14ARF, mdm2, p53 positive rates in NPC, CINE were found to be 31.0%, 41.4%, 65.5% and 100%, 0, 0, respectively. The expression of p14ARF protein were significantly degraded and mdm2, p53protein were significantly elevated in NPC when compared with CINE (P0.05). (2) Spearman correlation analysis showed that the expression of p14ARF, mdm2, p53 had no correlation in NPC (P0.05). (3) The expression of p14ARF, mdm2 and p53 had no relationship with patients lymph node metastasis, stage, gender, age(P0.05). (4) Kaplan-Meier analysis showed that the expression of mdm2 was significantly correlated with survival time in NPC (P0.05). p14ARF, p53 expression were irrelevant with the survival time(P0.05). Conclusion p14ARF, mdm2 and p53 may be involved i