类风湿关节炎滑膜成纤维细胞对T 淋巴细胞生物学行为的干预作用.pdfVIP

1092 Tianj in Med J , Nov 20 12, Vol 40 No 11 doi:10.3969/j.issn.0253-9896.2012.11.002 类风湿关节炎滑膜成纤维细胞对T 淋巴细胞生物学 行为的干预作用* 赵文君 崔爽爽 邢国胜 于顺禄 摘要 目的 探讨体外培养 风湿关节炎 RA冤患者滑膜成纤维细胞对T 淋巴细胞生物学行为的影响遥 方法 滑膜组织取自关节置换术的 RA 患者袁 酶消化法获得滑膜成纤维细胞并扩增培养袁T 淋巴细胞选用Jurkat T 淋巴 细胞株遥将T 淋巴细胞按培养方式分为单纯T 淋巴细胞培养组及与 RA 滑膜成纤维细胞共育培养组 2 组遥分别采 用噻唑蓝 MTT 冤法尧流式细胞仪和实时荧光定量 PCR 方法检测 2 组T 淋巴细胞的扩增能力尧凋亡 及白细胞介素 IL冤-2 mRNA尧IL-23p19 mRNA 的表达水平遥 结果 共育培养组 4 d 和7 d 时T 淋巴细胞增殖率高于单纯培养组袁 而10 d 和13 d 时增殖率低于单纯培养组袁差异均有统计学意义 P 0.01 冤曰共育培养组 24 h 和 48 h T 淋巴细胞 凋亡 均低于单纯培养组袁差异有统计学意义 P 0.05 冤曰共育培养组较单纯培养组T 淋巴细胞表达较高的白细 胞介素 IL冤-2 mRNA 2.33依0.09 vs 1.00依0.12冤和 IL-23p19 mRNA 4.01依0.25 vs 1.00依0.15冤袁差异均有统计学意 义 t 分别为 15.03 尧17.78 均P 0.01 冤遥 结论 RA 滑膜成纤维细胞可诱导T 淋巴细胞的激活袁这可能是 RA 发病及 进展的影响因素之一遥 关键词 关节炎袁 风湿 滑膜 成纤维细胞 T 淋巴细胞 淋巴细胞培养试验袁混合 Effects of Rheumatoid Synovial Fibroblasts on Biological Properties of T Cells ZHAO Wenj un 袁CUI Shuangshuang 袁XING Guosheng 袁YU Shunlu Researc h Ins titute of Tianj in Hospital, Tianj in 300211, China Abstract Obj ective: To investigate the effect of rheumatoid synovial fibroblast on biological properties of T cells. Methods: The sample of synovial tissue was obtained from rheumatoid arthritis (RA) patients with j oint replacement surgery. The synovial fibroblasts were extracted by enzyme. The Jurkat T cell line was used in the experiment. These T cells were divided into two groups, T cell alone group and co -culture group according to whether cells were co -cultured with RA fibroblast. MTT, flow cytometry and real-time PCR were used to detect the proliferation ability, the apoptosis and