乳糖酶生物黏附纳米微囊的制备及黏附性评价.pdfVIP

研究探讨 乳糖酶生物黏附纳米微囊的制备及黏附性评价 1,2 1* 2 2 贺红军 ，孔保华 ，孙承锋，姜竹茂 1.东北农业大学食品学院（哈尔滨 150030）；2.烟台大学化学生物理工学院（烟台 264005） 摘 要 采用复乳法制备生物黏附乳糖酶纳米微囊，以壳聚糖和卡波姆作为黏附材料，分别考察了 壳聚糖和卡波姆单独表面修饰以及协同表面修饰对纳米微囊理化性质的影响，采用外翻肠囊法考察 纳米微囊在离体大鼠小肠黏膜的滞留程度。相对分子质量为120 kDa、脱乙酰度为95% 的壳聚糖和 934P型号的卡波姆作为修饰材料制备的纳米微囊粒径大小均匀、Zeta 电位绝对值和包封率较高。体外黏 附试验结果表明，壳聚糖、卡波姆修饰的纳米微囊在小肠黏膜的滞留率显著高于未修饰纳米微囊，壳聚糖 /卡波姆(质量比为2 ∶1)协同修饰纳米微囊的黏附性最高，小肠黏膜滞留率可达(91.1±3.4)%。 关键词 乳糖不耐症；乳糖酶；生物黏附纳米微囊；壳聚糖；卡波姆 Preparation of Bioadhensive Lactase-loaded Nanocapsules and Evaluation of Its Bioadhension in Vitro 1, 2 1* 2 2 He Hong-jun , Kong Bao-hua , Sun Cheng-feng , Jiang Zhu-mao 1.College of Food Science, Northeast Agricultural University (Harbin 150030); 2.Chemistry and Biology College, Yantai University (Yantai 264005) Abstract The bioadhensive nanocapsules loaded with lactase were prepared by double emulsion method. Two types of bioadhensive materials, chitosans and carbopols were chosen. The effects of surface modification of nanocapsules with chitosan and carbopol, whether alone or combinatorial, on the physicochemical characteristics were investigated. The adhension of nanocapsules at the surface of intestinal mucous was evaluated by everted gut sac method in vitro. Nanocapsules with uniform particle size, high Zeta potential and encapsulation efficiency were obtained using chitosan (120 kDa, 95%) or carbopol (934P) as coating material. In vitro adhension test showed that, the retention ratio of nanocapsules coated with chitosan or carbopol was signifi cantly higher than that of uncoated one. A combinatorial coating of chitosan and carbopol with different ratios had more effective effect. Whe