猪神经干细胞分离培养的研究进展.pdfVIP

第十一届中南地区实验动物科技交流会论文集 猪神经干细胞分离培养研究进展 1 1 2△ 1△ 汪亦男 那顺巴雅尔 刘忠华 顾为望 （1 东莞松山湖明珠实验动物科技有限公司 广东 东莞 523808 ，2 东北农业大学胚胎工程实验室 黑 龙江 哈尔滨 150030 ） 【摘要】神经干细胞用于神经学临床修复和基础理论研究的前提是首先完成神经干细胞的体外分 离、培养、纯化并大量扩增。鼠、人、猪中都已成功分离出神经干细胞并已尝试用于动物神经系统损 伤等疾病的治疗，尽管在鼠和人上的研究很多，相对于鼠神经干细胞在神经学临床应用上的局限和人 神经干细胞在材料来源上的不便，猪作为神经干细胞临床应用和基础研究的模式动物有很大的潜力。 但关于猪神经干细胞体外分离培养的研究非常少，本文对这方面的研究进展做一综述。 【关键词】神经干细胞；分离；培养 Development of isolation and culture of porcine neural stem cells 1 1 2△ 1△ WANG Yinan , NASHUN Bayaer , LIU Zhong-hua , GU Weiwang (1Songshan Lake Pearl Laboratory Animal Sci.Tech.Co.,Ltd,Dongguan, 523808,Dongguan,China , 2Laboratory of Embryo Biotechnology of Northeast Agricultural University, 150030, Harbin, China) 【ABSTRACT 】 The premise of neural stem cells used for clinical therapy in neuroscience and fundamental research is to accomplish isolation , culture, purification and amplification in vitro firstly. Some attempts have been taken to use them for repairing injuries and curing diseases in nervous system of model animals with the success of neural stem cells isolation from mice, human beings and porcine. Although more research about neural stem cells from mice and human beings has been reported, porcine would become more desirable model animal on fundamental and exploratory development because of a basic limitation of neurological applications on the use of rodent cell cultures and scarce source of human neural stem cells. Some research has revealed the possibility that porcine neural