miR-27 b通过c-MET抑制胃癌细胞的生长、增殖及侵袭转移.pdfVIP

· 26 · miR一27b通讨 c—MET miR一27b通过 c—MET抑制胃癌细胞的生长、增殖及侵袭转移 李丹波，徐细明 miR一27binhibitsgastriccancercellgrowth，invasionandmetastasisviatargeting c—MET LiDanbo．XuXiming DepartmentofOncologyCenter，WuhanUniversityPeopleHospital，HubeiWuhan430060，China． A【bstract】 Objective：TofindoutwhichmiRNAcanregulatec—METingastriccancer，andinvestigatewhetherit caninhibitgastriccancercellrgowth，invasionandmetastasisviatargetingc—MET．Methods：miRNAswhichcan regulatec—-METingastriccancerwerescreenedoutbypredictiontools．TheexpressionofmiRNAsingastriccancer celllineswasdetectedbyreal—timePCR．Theexpressionofc—METwasdetectedbywesternboltafteroverexpres— sionofmiRNAs．WeidentifyamiRNAasadirectregulatorofc—METexpressionbyluciferasereporterassay．Thea— bilityofgastriccancercellgrowth，invasionandmetastasiswasinvestigatedbyplatecloningandtranswellmigration assaysafteroverexpressionofmiRNA andc—MET．Results：miR一34a．miR一27bandmiR一31mayreuglatec— MET ingastriccancer．miR一34a，miR一27bandmiR一31expressionwasdecreasedingastriccancercelllinescon— paredtonormalgastricmucosacelllines(P0．05)．miR一27bcouldsignificantlyattenuatec—METexpression， andmiR—-27basadirectregulatorofc—-MET expressionbyluciferasereporterassay．Theabilityofgastriccancer cellrgowth，invasionandmetastasiswasdecreasedafteroverexpressionofmiR一27b，rescueofc—METexpression reversedmiR一27bsuppressionofmigrationandinvasion．Conclusion：miR 一27bcancurbc—MET expressionby bindingc—MET3 UTR，andmiR 一27bcaninhibitgastriccancercellgrowth，invasionandmetastasisviatargetingc — MET． K【eywords】miR一27b；c—MET；gastriccancer ModernOncology2015，23(01)：0026—0031 【摘要】 目的：找出调控胃癌细胞 c—MET的miRNA，研究该 miRNA能否通过c—MET抑制 胃癌细胞的生 长、增殖及侵袭转移。方法：预测软件筛选出可能调节 胃癌 c—MET的miRNA。荧光定量PCR检测 胃癌细胞 株 (N87、MKN45、AGS)及正常胃黏膜细胞(GES1)中该miRNA的表达水平。过表达miRNA后检