标记人血清抗体IgG胶体金免疫法对不同标本幽门螺杆菌抗原的测定.pdfVIP

()2009 2 3 1 Ch ni J Exp Clin InfectDis (Electronic Edition), Februar 2009, Vo l3, No. 1 1 IgG 贾克东 丁元军 刘东林 刘晖 罗斌 ! (H. p lor,i HP) ( 1)H. p lori, HP IgG; ( 2) , HP, ; ( 3) HP, , HP 37∀ , HP 3 60HP , 69. 04% , , 57. 14% , , 40. 48% ; 61. 67% , HP 65% HPHP, , , ! ; ; Detection of Helicobacter pylori antigen with goldlabeled IgG purified from Hpylori infected patients JI A K edong, DI NG Yuanjun, LIU D onglin, LIU H ui, L UO B in. D ep artm ent of Gastroenterolog , J iangx iP rovincialP eop le# sH osp ital, Nanchang 330006, China Corresp ond ing author: JIA K edong, Email: j iakedong@ tom. com Abstract! Objective To approach the feasib ilit and accurac to detectH e licobacter p lori antigen in different samples b colloidal gold mi munoassa . Methods ( 1) The sera w ith high titer antiH. p lori antibod were gathered. IgG w as purified and enriched through ammon ium sulfate precip itation and protein A resin affin it chro m atograph . ( 2) The colloidal gold w ere prepared w ith citrate trisodium reduction m ethod. Purified human antiH. p lori IgG w as labled w ith colloidal gold, wh ich nam ed goldtaggedantibod . ( 3) Purified hum an antiH. p lori IgG w as fixed on the : : 330006 , () ; () : , Em ail: jiakedong@ tom. com 2 () 2009 2 3 1 Ch ni J Exp Clin InfectDis (Electron icEditoin), Februar 2009, Vol 3, No. 1 m embrane of n itrocellulose. Samples of gastric ju ice, feces and seraw ere added on the m embrane where antigenantibod comb ined, respectivel. The result was shown b goldtaggedantibod and w ith red spot on the n itrocellulose m embrane when consid ered as positive sample. Results The samples of gastric ju ice, feces and serum of 60 patients w ere detected forH. p lori antigen b H. p lori antigen mi munegold test, the accurac of gastric ju ic