溶菌酶热变性的DSC研究.pdfVIP

物理化学学报(Wuli Huaxue Xuebao) 84 A cta Phys. Chim. Sin., 2007, 23(1) ：84-87 January [Article] 溶菌酶热变性的DSC 研究 盈盈1 胡新根1,* 于 丽2 李文兵1 林瑞森3 (1 州大学化学与材料科学学院, 浙江温州 325027; 2 山东大学胶体与界面化学教育部重点实验室, 济南 250100; 3 浙江大学化学系, 杭州 310027) 摘要: 用差示扫描量热法研究了固体溶菌酶的热变性以及水溶液中不同变性剂与浓度对溶菌酶变性的影响. 结果表明, 溶剂水的存在及变性剂尿素和盐酸胍的加入使溶菌酶的变性 度降低, 变性焓减小; 同时, 在一定的 浓度范围内, 溶菌酶的变性 度和变性焓随变性剂浓度的增大而降低. 盐酸胍的变性效果较尿素强, 这是由 盐 酸胍与蛋白质分子间除了氢键作用外还存在着静电作用. ： 关键词 溶菌酶; 尿素; 盐酸胍; DSC; 热变性 ： 中图分类号 O642 Study on Thermal Denaturation of Lysozyme by DSC FANG Ying Ying1 HU Xin Gen1,* YU Li2 LI Wen Bing1 LIN Rui Sen3 1 ( School of Chemistry and Materials Science, Wenzhou University, Wenzhou 32502 7, Zheji ang Province, P. R. China; 2Key Laboratory f or Colloid and Interf ace Chemistry of the Ministry of Education, Shandong University, Jinan 250100, P. R. China; 3Depa rtment of Chemistry, Zhej iang University, Hangzhou 310027, P. R. China) Abstract: The thermal denaturation of solid lysozyme and the effects of denaturants and their concentrations on denaturation of lysozyme in aqueous solutions were studied by differential scanning calorimetry (DSC). The results showed that both denaturation temperature and denaturation enthalpy of lysozyme decreased as the existence of water and the addition of urea and guanidine hydrochloride (GuHCl). In addition, denaturation temperature and denaturation enthalpy decreased as the concent