甘草甲醇提取物FM100对小鼠溃疡性结肠炎的药效学的研究.docVIP

甘草甲醇提取物FM100对小鼠溃疡性结肠炎的药效学研究 作者:吴国泰,任远,马骏,王志旺 【摘要】 目的 观察甘草甲醇提取物FM100对小鼠溃疡性结肠炎的作用。方法 用三硝基苯磺酸钠（TNBS）和乙醇制造小鼠溃疡性结肠炎模型，空白对照组和TNBS模型组灌胃等容量生理盐水，阳性对照组灌胃地塞米松0.2 mg/kg，FM100大、中、小剂量组分别灌胃FM100 300 mg/kg、200 mg/kg、100 mg/kg,连续灌胃14 d。观察小鼠体质量变化、腹泻、结肠黏膜组织形态学、脾脏指数及髓过氧化物酶(MPO)活力等指标。结果 甘草甲醇提取物FM100可不同程度地改善TNBS/乙醇所致溃疡性结肠炎小鼠体质量下降和腹泻症状，明显降低结肠黏膜粘连及其溃疡分值和脾脏指数，但可增高MPO活力（P0.05或P0.01）。结论 甘草甲醇提取物FM100对小鼠溃疡性结肠炎有较好的改善作用，能减轻结肠局部的病理性损伤。 【关键词】 甘草甲醇提取物FM100；溃疡性结肠炎；小鼠 　　The pharmacodynamics study of methanol extract of Glycyrrhizae FM100 on ulcerative colitis 　　Wu Guotai, Ren Yuan, Ma Jun, Wang Zhiwang, Shao Jing,Jiang Xiaoxia 　　Gansu College of Traditional Chinese Medicine,730000,Lanzhou,China Abstract:Objective To investigate the effects of methanol extract of Glycyrrhizae FM100 on ulcerative colitis (UC) mice. Methods A mouse model of UC were made by TNBS and alcohol. The blank control group and model group were given NS, the positive group was taken 0.2 mg/kg Dexamethasone, FM100 large, middle, small groups were taken 300 mg/kg, 200 mg/kg, 100 mg/kg FM100 separately for 14 days. Observed body weight, diarrhea, histomorphology of colon mucosa, spleen index and myeloperoxidase (MPO) activity. Results Methanol extract of Glycyrrhizae FM100 could improve in different degrees the decreased body weight and diarrhea symptom, decreased the colon mucosa adhesion, ulcer grade and spleen index, increased the MPO activity. Conclusion FM100 can improve the UC of mice, alleviated the pathologic injure in partial colon. Key words: methanol extract of Glycyrrhizae FM100; UC; mouse 溃疡性结肠炎（uicerative colitis，UC）是以腹泻、黏液脓血便、腹痛和里急后重为主要症状，以结肠黏膜慢性炎症和溃疡为病理特点的一种消化道疾病，近年来发病率呈上升的趋势，临床缺乏有效的治疗手段和药物。有文献报道甘草甲醇提取物FM100具有抗消化性溃疡的作用[1],但对UC有无疗效尚未见报道。本实验研究观察了甘草甲醇提取物FM100（含甘草甜素较少的精制部分）对小鼠溃疡性结肠炎的治疗作用，为临床用药提供实验依据。 　　1 实验材料 　　1.1药品与试剂 甘草甲醇提取物FM100，参考文献[2]方法制备；地塞米松片，国药准字浙江仙琼制药有限公司生产，批号：040904；三硝基苯磺酸钠（TNBS），天津市福晨化学试剂生产厂生产，批号髓过氧化物酶（MPO）试剂盒，南京建成生