携qnrA基因肠杆菌科细菌的耐药性及其分子机制研究.pdfVIP

778 Chin J Nosoco iol Vol. 17 No.7 2007 qnrA 1 2 1 2 1 3 吴创鸿 , 陆 坚 , 邓启文, 张国良 , 肖 潇 , 张扣兴 ( 1. , 518052; 2. , 518020; 3. , 510630) : qnrA 2003- 2004 332 , PCR qnrAgyrA parC ( ESBLs) A pC ; KB Etest qnrA , ESBLs qnrA 3.9%, ( 15. 6%) ;qnrA , ESBLs gyrA parC ; qnrA Sul1 In37 InX qnrA , ,, : ; ; ; : R378. 2 : A :2007) Antimicrobial Agents Resistance and Its MolecularMechanisms of Clinical Isolates of Enterobacteriaceae with qnrA * WU Chuanghong , LU Jian, DENG Qiwen, ZHANG Guoliang, XIAO Xiao, ZHANG Kouxing ( * T he S ix th P eop le!s H osp ital of Shenz hen, Shenz hen, Guang dong 518052, China) Abstract: OBJECTIVE T o deter ine the prevalence of gene qnrA in clinical isolates of Enterobacteriaceae, phenotypes of drug resistance and their olecular echanis s. MET ODS Totally 332 isolates of Enterobacteriaceae fro Shenzhen City fro 2003 to 2004 were screened for the presence of qnrA by PCR. T he gyrA, and parC genes the genes, coding ESBLs and A pC were a plified and sequenced. KB ethod, the international standard plate dilute ethod and Etest were used to detect MIC of the isolates with qnrA. ESBLs were distinguished by the phenotypic confir atory test. RESULTST he incidence of qnrA in clinical isolates was 3.9%, thehighest incidence of 15.6% occurred in Enterobacter clo acae. T he clinical isolates were ultiresistant. gyrA And parC utation associated with quinolone resistance and ESBLs producing was confir ed in ost of the isolates with qnrA. qnrA Was e bedded in In37 or InX classified to Sul1type class integrons. CONCLUSIONS Gene qnrA located in plas ids is a