正常大鼠静脉注射PEG包裹胰岛素脂质体的降血糖作用_英文_.pdfVIP

28 Journal of Chinese Pharmaceutical Sciences 2004 , 13 ( 1) Hypoglycemic Effect of Intravenous Polyethylene Glycol - Coated Liposomal Insulin on Normal Rats ZHAN G Xuan , ZHAN G Hua , WAN G Guiling , ZHAN G Dawei , WAN G Jing , L IN Wei , and ZHAN G Qiang ( Dep artment of Pharmaceutics , School of Pharmaceutical Sciences , Peking University , B eij ing 100083 , China) Abstract : Aim 　To evaluate liposome as an inj ectable delivery system of proteins , insulin was chosen as model drug and the hypoglycemic effect of PEGcoated liposomal insulin was tested. Methods 　The PEGcoated liposomal insulin was prepared by reversalphase emulsion evaporation . For pharmacodynamic study , insulin (25 IU ·kg- 1 ) was intravenously administered in phosphatedbuffered saline (PBS) solution , conventional liposomes , and PEGcoated liposomes , separately , to normal Wistar rats. Blood glucose levels were determined by the glucose oxidase method. Results 　The mean diameter of the PEGcoated liposomal insulin was 58. 4 nm , while the encap sulation ratio reached 18. 33 %. After intravenous adminis tration of insulin solution , insulin liposome , and PEGcoated liposomal insulin , the minimum blood glucose concentrations ( C %) reached 25. 26 ±5. 75 % , 33 . 92 ±12 . 42 % , and 42 . 39 ±105 % of the initial level , respectively , and the time min periods to reach the minimum blood glucose level ( T ) wer