耐亚胺培南铜绿假单胞菌_内酰胺酶分布分析.pdfVIP

2011 21 1 Chin J Nosocomiol Vol.21 No. 1 2011 139 1 2 2 2 2 高巍 , 陈刚 , 宋阳 , 杨继勇 , 张有江 ( 1. 211 , 150001; 2. , 100853) : (IM P)( PAE) KB , (DDST ), ( PCR) 2008 68 ,DDST 12; 2009 50 ,DDST 11 ; 2008 465 PAE KB IMP 14.8% MIC 14. 6%, 2009 285 PAE KB IPM 18.9%, MIC 17. 5%; , IM P 2. 5%VIM 5.9%GIM 0. 8%KPC 4.2%XA10 28. 8%XA2 7.6%XA1 1.6% , IM P PAE , XA ESBLs : ; ; + : R378. 99 1 : A : 2011) Distribution of lactamases among clinical imipenemresistant p seud omonas aerug inosa * GA Wei , CHEN Gang, S NG Yang, YANG Jiyong, ZHANG Youjiang ( * Chi nese P L A T he 211 H osp it al, H arbi n, H e il ongj iang 150001, Ch ina) Abstract: OBJECTIVE To investigate the antimicrobials susceptibility and prevalence of lactamases for imipenem ( IMP )resistant Pse udomonas aer ug inosa ( PAE) . METHODS Drug susceptibility test was performed by agardilution and KB method. Mellolactamases (M BL) were detected by double disc synergistic test ( DDST) . Resistance determinants were screened by PCR amplification using specific primer for each kind of enzymes. RES LTS 12 and 11 isolates were positive for MBL DDST among 68 and 50 nonduplicated strains of clinical imipemenresistant P . aer ug inos a isolated in 2008 and 2009, respectively. T he resistant rate of clinical P . aer ug i nosa isolates was 14.6% and 17. 5% according to MICs. Resistance genes distributed as follows: IMP 2. 5%, VIM 5.9%, GIM 0.8%, KPC 4.2%, XA10 28.8%, XA2 7. 6%, XA1 1.6%. CONCL SION T he resistance rate of common used antibiotics is increasing in clinical P . aer ug inosa isolates.