04 反相原理篇.pptVIP

Title or job number 反相层析 Reversed Phase chromatography 内容 内容 内容 Purification of Dye667-labelled 20-mer oligonucleotide 多肽/寡核苷酸等的分析与分离 谢 谢！ Speaker′s notes Reversed Phase Chromatography - how does it work? To give an example of that we are going to look at some pictures which will show medium beads as indicated on this picture. These medium beads have a lipophilic layer made up of ligands. What these ligands are and how they are composed we are going to look into a little bit later on. This is to give an example of the principles of Reversed Phase Chromatography. In these examples we are