恶性肿瘤化疗多药耐药动物模型的实验研究_王熙才.pdfVIP

　 2006 4 11 4 　Ch nese C ln ca l Onco logy, A pr. 2006, V o.l 11, N o. 4　 2 83 650 118　昆明　云南省肿瘤医院肿瘤研究所抗肿瘤药物研究室 王熙才, 蒋永新, 伍治平, 金从国, 谷玉兰, 陈晓群, 陈　艳 　　【　】　:, 、 。:, BA BL /c, S- 180 , , 15, S-180R。: 66, VP-16 9;: P-170 ; 89 。: S-180R , 、。 　　【】　;　S-180 ;　;　VP-16 :R 730.53　　:A　　:1009 - 0460(2005)06 - 02 83 - 04 Experimental research of the multidrug resistant animalm odel for malignant tumor chemotherapy 　　WANGX i-ca,i JIANG Yong-xin, WU Zh-ip ing,JIN Cong-guo, GU Yu-lan, CHEN X iao-qun, CHEN Yan. Tum or Institut of Yunnan P rovinc, K unm ing 650118, China 　　【Abstract】　Objective:To deve lop the mu lt drug res stant an m al m ode l fo r m a lgnan t tum or chem othe rapy and se lect drug o r ra se som e othe r the rapeut c strateg e s fo r revers ng mu lt drug res stance in v ivo. M ethods:T he BA BL /c m ce no cula ted by S-180 ce lls w ere njected w th adr am yc n n low dosage fo r 15 cyc les. T he mu lt drug re s stant an m al m ode l and res stan t ce lls S-180R w as a- ch eved. Results:The Ad r amy c n o f these cellsw as 66 tm esm o re than the r parent ce lls. The res stance to a typ ca lDNA topo some-r a se Ⅱ nh b to r V P-16(E topos de)w as ncreased 9 tm es than the r parent cells. O verexpre ss on o f m ult drug res stant gene (M DR gene)produc t P-g ly coprote n w as also dem onstra ted by mm unoh sto chem ca lm e thod. Furthe rmo re, the ab l ty o f the res stant ce lls to reduce ne t cellu la r drug accumu la t on, w h ch w as m easured w th flow fluorescence cy tom e try, w as 89 tm es h ghe r than the r paren t ce lls. A ll of the se conf rm ed tha t the res stance to ad r amy c n of S-180R cellsw ere m a nly due to the overexpress on of p-g lycop ro te n. Conclusion:These new deve loped S-180 ce lls and mou se anm a lm ode lw ould be u seful to se lect drug o r ra se som e o the r the rapeut c stra teg es fo r reve rs n