G3BP在喉鳞状细胞癌的表达及其临床意义.docVIP

G3BP在喉鳞状细胞癌的表达及其临床意义 　　作者：徐志坚，彭解人，黄晓明 作者单位：1. 中山大学附属第二医院耳鼻咽喉科， 广州 510120; 2. 惠州市中心人民医院， 广东 惠州 516001 　　【摘要】 　　目的 研究GTP酶激活蛋白SH3功能区结合蛋白(G3BP)在喉鳞癌组织中的表达情况及其与喉鳞癌生物学行为之间的关系。方法 采用免疫组织化学方法检测91例喉鳞癌组织中G3BP的表达情况，并探讨他们与喉鳞癌的肿瘤组织分化程度、TNM分期、淋巴结转移和预后的关系。结果 G3BP在喉鳞癌的阳性表达率为84.7%。其表达水平与肿瘤分化程度(P=0.008)、临床分期(P=0.005)、淋巴结转移(P=0.000)及肿瘤复发转移(P=0.000)均有密切关。G3BP表达水平越高，患者术后生存时间越短(P=0.000)。结论 G3BP在喉鳞癌表达与肿瘤分化程度、临床分期、淋巴结转移、肿瘤复发转移情况及患者的预后有密切关系。G3BP可作为判断喉鳞癌患者预后的有效参考指标。 　　【关键词】 喉鳞状细胞癌 GTP酶激活蛋白SH3功能区结合蛋白 免疫组织化学 　　in laryngeal squamous cell carcinoma and it clinical significance 　　XU Zhijian1,2，PENG Jieren1, HUANG Xiaoming1, GUAN Zhong1, ZHU Xiaofen2, LIAO Yanping2 　　(1. Department of Otolaryngology, Affiliated Second Hospital of Sun Yetsen University, Guangzhou 510120, China; 　　2. Huizhou Central People′s Hospital, Huizhou 516001, Guangdong, China) 　　To investigate the expression of the RasGTPaseactiviting protein SH 3 domain binding protein(G3BP) in laryngeal squamous cell carcinoma(LSCC) and to explore its correlation with biological behavior. Methods The expressions of G3BP in 91 cases of LSCC were determined by immunohistochemistry. The relationships between protein expression and tumor size, differentiation degree, TNM stage, lymph node metastasis and prognosis of LSCC were explored. Results The positive expression rate of G3BP in LSCC was 84.7%. The G3BP expression level was correlated with the degree of differentiation of tumors(P=0.008), clinical stage(P=0.005), lymphatic metastasis(P=0.000),　and tumor recurrence or distant metastasis(P=0.000), and also the expression of G3BP was significantly related to survival in a negative timedependent manner in LSCC patients(P=0.000). Conclusions The expression of G3BP is related to the degree of differentiation of tumors， clinical stage, tumor recurrence or distant metastasis, lymphatic metastasis and survival in LSCC patients. Overexpression of G3BP may be used as a prognostic marker for overall survival of LSC