蚕沙叶绿素的提取和性质的研究.pdfVIP

华东·华中地区第十二次蚕种学术研讨会论文集 蚕沙叶绿素的提取及性质的研究 陈芳艳 方平 林健荣 （华南农业大学动物科学学院, 广东 广州 510642 ） 摘 要：研究以丙酮为溶剂超声波辅助萃取法提取蚕沙叶绿素的工艺，并将该方法与传统提取法、 二氧化碳超临界萃取法进行了比较，最后对蚕沙叶绿素的部分理化性质进行了研究。结果表明， 以丙酮为溶剂,超声波辅助萃取法提取蚕沙的最佳工艺为：以粉状蚕沙 1 （g ）: 丙酮 2.5 （mL ） 浸提，萃取 3 次，每次 45 min ，该条件下叶绿素的提取率为7.63 mg 叶绿素/g 蚕沙，提取率比另 外两种方法都高；叶绿素耐光、热及氧化还原剂的稳定性差。 关键词： 蚕沙 叶绿素提取 理化性质 中国分类号 S886.9 文献标识码 A Extraction and Properties of Chlorophylls from Silkworm Excrement Chen Fang-Yan Fang Ping Lin Jian-Rong (College of Animal Science, South China Agricultural University, Guangzhou,510642,China) Abstract: Chlorophylls in silkworm excrement were extracted with acetone using ultrasonics method. Then results of three extraction methods, ultrasonics, traditional method and supercritical carbon dioxide extraction,were compared. Last the physical and chemical properties of silkworm excrement cholrophylls were studied. The result showed that the optimum technological parameters of chlorophyll extraction with acetone using ultrasonics were as follws: silkworm excrement power, the solid-liquid ratio was 1 （g ）: 2.5 （mL ）, the extraction times were 3 ，45 min each time. petroleum ether was 1 ∶3.The extration rate was 7 。63mg chlorohgylls/g silkworm excrement, which was higher than the other two methods, traditional method and supercritical carbon dioxide extraction. The stability of silkworm excrement chloroghylls were bad in the conditions of light, heat and oxidation-reduction. Key words: Silkworm excrement; Chlorophyll extraction; Physical and Chemical Properties 蚕沙俗名蚕粪，是桑蚕摄食桑叶后的排泄物。蚕沙不仅含有丰富的粗蛋白和碳水化合物 等多组分物质，而且还是提取叶绿素、叶黄素、类胡萝卜素、植物醇、叶蛋白、果胶等来源 丰富