普萘洛尔分子印迹整体柱的制备及其结合特性研究.pdfVIP

普萘洛尔分子印迹整体柱的制备及其结合特性研究 张倩倩，傅 强，艾力江·阿木提 （西安交通大学医学院药学系，西安 710061 ） 摘要 目的：合成普萘洛尔分子印迹整体柱并研究聚合物的结合特性。方法：采用原位聚合 法，以普萘洛尔为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯作为交联剂， 甲苯和十二醇混合溶液为致孔剂，合成普萘洛尔分子印迹整体柱。通过 Scatchard 分析对聚 合物的结合特性进行研究。结果：普萘洛尔分子印迹整体柱的最佳聚合条件为：模板分子、 功能单体之间的比例为 1: 4，交联度为 85%，甲苯含量占整个致孔剂体系的 18%。Scatchard 分析表明聚合物对普萘洛尔的识别主要有两类结合位点，其离解常数、最大表观结合量分别 为：3.836 × 10-5 -3 -1 -1 和 1.026 × 10 mol·L ；69.15 和 263.63 μmol·g 。结论：合成的印迹聚合 物对模板分子具有很强的亲和力和特定的选择性，且具有较大的结合容量，可望将其应用于 普萘洛尔的体内药物分析中。 关键词：普萘洛尔；原位法；分子印迹整体柱 Preparation of Propranolol Molecularly Imprinted Monolithic Column and Study on its Bonding Speciality * ZHANG Qian-qian, FU Qiang , Elijan·Amut (Faculty of Pharmacy, Medical School of Xi’an Jiaotong University, Xi’an 710061, China) Abstract Objective: To prepare propranolol (PL) molecularly imprinted monolithic column and to study its bonding speciality. Method: A propranolol molecularly imprinted monolithic column was prepared by in situ technique, using propranolol as template molecule, methacrylic acid (MAA) as a function monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linker, and toluene and dodecanol as porogenic solvents by in situ technique. The bonding speciality was studied by Scatchard analysis. Results: The optimal polymerization conditions were as follows: the proportion of template-monomer was 1: 4; the degree of cross linking was 85%; the percentage of toluene in porogenic solvents was 18%. Scathcard analysis showed that two classes of binding sites existed in the PL-imprinted polymers, with their Kd a