植物转基因整合座位的结构.pdfVIP

HEREDITAS (Beijing) 2007 2 , 29(2): 157―162 ISSN 0253-9772 综述 DOI: 10.1360/yc-007-0157 1 2 2 3 1 陈建民 , 姚泉洪 , 熊爱生 , 马鸿翔 , 洪义欢 1. , 225009; 2. , 201106; 3. , 210014 : 通过农杆菌和直接 DNA 转移技术所获得的转基因植株都具有复杂的转基因座位, 转基因整合染色体和 染色体区段是随机的, 但组织培养的选择作用表现为非随机性, 偏向整合于染色体的基因富集区。转基因座位 除少数含有完整的单拷贝转基因之外, 大多数转基因座位中外源转基因片段、基因组片段和填充 DNA 相间而 存在。转基因座位中转基因及基因组 DNA 片段产生缺失、重复和染色体的重排, 转基因的完整性对转基因表 达具有重要作用。 : 农杆菌介导; 直接 DNA 转化; 转基因座位; 染色体重排; 旁侧序列 Structures of transgenic integration loci in transformed plants CHEN Jian-Min 1 , YAO Quan-Hong 2 , XIONG Ai-Sheng 2 , MA Hong-Xiang 3 HONG Yi-Huan1 1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; 2. Agro-Biotechnology Research Center of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3. Institute of Genetics and Physiology Jiangsu Academy of Agricultural Science, Nanjing 210014, China Abstract: The plants transformed by either Agrobacterium-mediated or directly delivered DNA transformation possess complex transgenic loci. The transgenes tend to be integrated into gene-rich regions on chromosomes due to selection of transgenic cells during tissue culture rather than integrated elsewhere randomly. Fragments of transferred DNA are inter- spersed with genomic DNA and filler DNA at most of transgenic loci except a few which contain complete single-copy of transgene. Analysis of transgenic loci flanking sequences indicated that deletion, duplication and chromosomal rearrange- ment often occurred for transgene and genomic DNA. The complete transgenic structure is important for its expression in transformed plants. Keywords: Agrobacte