四个中国地方鸡种TLR4基因第2内含子多态性差异分析.pdfVIP

四个中国地方鸡种TLR4基因第2内含子的多态性差异分析 胡艳 陶志云单艳菊陈宽维李慧芳 江苏省家禽科学研究所，扬州 225125 摘要：为探究仙居鸡、白耳鸡、清远麻鸡和安卡鸡四个不同鸡种TLR4基因的多态和群体遗传特 性。本研究采用PCR-SSCP和DNA测序的方法检测了TLR4基因第2内含子的多态性。结果表明，四 个鸡种TLR4基因的第2内含子上均发现了G1894C的突变，共产生了AA、AB和BB三种基因型，四个 鸡种该位点的多态信息含量(PIC)分别为0.570、0.597、0.583和0.588，均属于高度多态性且处于Hardy- Weinberg平衡状态(P＞0.05)。四个品种鸡G1894C多态位点的基因型在感染鸡白痢沙门氏菌阳性组和 阴性组间的分布差异均不显著(P＞0.05)。G1894C多态位点在仙居与白耳两个品种间基因型分布差异 极显著(P＜0.01)。研究结果为进一步开展鸡抗病相关基因研究提供了一定的理论基础。 关键词：鸡；TLR4基因；单核苷酸多态性 Polymorphismanalysesofintron2ofTLR4gene amongfourKindsofChineseNativeChickens HU Yan CHENKuanwei TAOZhiyunSHANYanju LIHuifang KeyLaboratoryofPoultryHeredityBreeding,InstituteofPoultryScienceof JiangsuProvince,Yangzhou,Jiangsu 225003,China Abstract:Thegeneticdistributionofthepolymorphismsofintron1ofTLR4geneinfour differentchickenbreedsincludingXianju,Baier,Qingyuan,AnkachickenwastestedbyPCR— SSCP andDNAsequencinginthisstudy.TheresultindicatedthataG1894Cmutationexistinthe4 differentchickenbreeds.Thereexistthreegenotypes(AA,AB,BB)inallthebreeds;Theresult showsthatinspectedlocuswashighdiversity,theaveragepolymorphisminformationcontent(PIC)of fourwasXianjuchicken0.570,BaierChicken0.597andQingyuanchicken0.583,Ankachicken 0.588respectively.ThedistributionofChTLR4phenotypeinthebreedswasaccordedwiththelaw ofHardy— Weinbergequilibrium.ThegenotypesofG1894Cpolymorphismlociisnosignificant differencesbetweenthepositiveandnegativegroupsafterchickensinfectedwithSalmonella pullorum(P＞0.05).GenotypedistributionofG1894Cpolymorphismlociwashighlysignificant differencesbetweenthexianjuandthebaier,whichwillprovidescientificbasisandtechnological supportingforresistance-relatedgeneanalysisinthefuture. Kewwords:Chicken;TLR4;SingleNucleotidePolymorohisms;PCR-SSCP ◇实验研究◇ Toll样受体4(ToIl—likereceptor