P38MAPK激活在高血糖致大鼠微血管通透性增高过程中的作用.pdf

2006 9 Septem ber 2006 26 9 Basic C lin ica lM edicine V o l. 26 No. 9 : 1 00 1-6 32 5 2 006 ) 0 9-0 94 7- 05 P38 MAPK * 朱艳军, 李 强, 陈 波, 黄绪亮, 黄巧冰 , 5 105 15) : P38 M APK SD , ST Z 55 mg / kg ) MKK 6b A ) MKK 6b E): ; MKK 6b A ): , 24 h MKK 6b A ) 25 mL / kg; MKK 6b E): 24 hM KK 6b E) 25 mL / kg ; ; M KK 6b E) , MKK 6b A ) P38 MA PK : ; ; ; ; P38 M APK : R 5871 : A Ro le o f P 38 MAPK activation in g lucose- induced m icrovascu lar hyperperm eab ility of rats * ZHU Y an-jun, L I Q iang, CH EN Bo, HUANG Xu- liang, HUANG Q iao-b ing D epartm ent of Pathophy sio logy, Sou thern M ed ical U n iversity, Guangzh ou 505 15, Ch ina) Abstract: Objective T o study the ro le o f P38 m itogen-act ivated prote in k inase P38 MA PK ) activation in high level g lucose- induced m icrovascu lar hyperperm eab ility. M ethods Rats w ere induced to diabetis by intraper itonea l in ject ion of streptozotoc in STZ ). The ratsw ere d iv ided into 5 group s, including norm al, d iabetes, control, MKK6b A ) and MKK6b E ) g roup s. The perm eab ility coeffic ient to a lbum in P a) w as m easured in venu les o f in v ivo m esenterium u sing a fluorescence ratio techn ique; M orpholog ical changes of m icrovascu lar endothe lial cellw ere m o- n ito red by observ ing fluo rescence of F-actin sta ine