红豆杉内生真菌代谢产物的分离与鉴定.pdfVIP

第 30卷 第 5期 Vo.l 30 No. 5 2010年 5月 Jou rn a l of C entra l South U n iv er sity of For estr y T echn ology May 2010 , , , (中南大学化学化工学院, 湖南长沙 410083) : 从红豆杉树皮中分离到了一株能产生物活性化合物的青霉菌, 并对该菌株的发酵产物进行了分离和分析, 从中提取分离到了 2种化合物。经过图谱分析和文献比对,确定这 2种化合物的结构为过氧麦角甾醇和贝诺酯。过 氧麦角甾醇具有抗肿瘤、抑菌等活性, 贝诺酯是一种应用广泛的镇痛消炎药, 本实验中产量分别为2和 11 5mg/L。其 他成分经 GC/MS分析主要为十三酸、十八烯酸等脂肪酸化合物。本实验是首次从植物内生真菌中分离得到贝诺酯, 也是天 贝诺酯的首次报道。 : 微生物学; 内生真菌; 南方红豆杉; 贝诺酯; 过氧麦角甾醇 : Q 93; Q 949. 32 : : 1673) 923X(2010)05) 0101) 05 Study on m etab olites fr om an en dophyte of Taxu s yunn an esis YU N Yao, LIU Jia2jia, CHEN Shu2juan, W NG Xiao2jun ( SchoolofChem istry, CentralSouthUniversity, Changsha 410083, Hunan, China) Ab str ac t: strain ofP enn icillium spp. was isolated from barks ofT axu s yunnan esis as an endophyte. Metabolite of itwas separated and analysed. Two bioactive compoundswere isolated from the extract of fungusmycelium by silica gel chromatogra2 phy. The structureswere elucidated to be ergosterolperoxide and benorilate on thebasisofdetailed spectroscopic analysis and comparison with the literatures. Ergosterolperoxide is on anti2tumor and anti2microbial active compound, and benorilate is a kind ofwidelyused anti2inflammatory drug. Theyield is 2. 0 and 1. 5 mg/L. Other componentswere elucidated tobemainly n2Tridecanoic acid, 92Octadecenoic acid and some other fatty acid based on GC/MS analysis. It is the first report ofnatural source ofbenorilate, especially from plant endophyte. K ey w ord s: m icrobiology; benorilate; T axu s yunn an esis; ergosterolperoxide; endophyte 南方红豆杉特产于中国,是国家Ñ级保护植物。 到了能产紫杉醇的内生真菌[2- 3] 。开发利用重要经