Intramyocellularlipiddropletsincreasewithprogression：肌细胞内脂滴增加与进展.pdfVIP

J Cachexia Sarcopenia Muscle (2011) 2:111–117 DOI 10.1007/s13539-011-0030-x ORIGINAL ARTICLE Intramyocellular lipid droplets increase with progression of cachexia in cancer patients Nathan A. Stephens Richard J. E. Skipworth Alisdair J. MacDonald Carolyn A. Greig James A. Ross Kenneth C. H. Fearon Received: 6 January 2011 /Accepted: 11 May 2011 /Published online: 31 May 2011 # The Author(s) 2011. This article is published with open access at S Abstract Results Compared with controls, cancer patients had in- Background Intramyocellular lipids are an important source creased numbers of lipid droplets (mean (SD) 1.8 (1.9) vs. of fuel for mitochondrial fat oxidation and play an 6.4 (9.1) per ×2,650 field, respectively,p =0.036). Mean (SD) important role in intramuscular lipid homeostasis. We lipid droplet diameter was also higher in cancer patients hypothesised that due to the phenotype associated with compared with controls (0.42 (0.13) vs. 0.24 (0.21) μm, cancer cachexia, there would exist an association between p =0.015). Mean lipid droplet count correlated positively with increasing weight loss and the number/size of intramyocel- the severity of weight loss (R =0.51, p =0.025) and negatively lular lipid droplets. with CT-derived measures of intermuscular fat (R =−0.53, Methods Nineteen cancer patients and 6 controls undergoing p =0.022) and visceral fat (R =−0.51, p =0.029). surgery were recruited. A rectus abdominis biopsy was Conclusions This study suggests that the number and size of performed and processed for transmission electron microscopy intramyocellular lipid droplets is increased in the presence of (TEM). Th