鱼降压肽的大孔吸附树脂脱盐及理化性质.pdfVIP

158 2009, Vol. 30, No. 24 食品科学 ※工艺技术 鱼降压肽的大孔吸附树脂脱盐及理化性质 1 1 1,2 陈季旺 ，孙 勤 ，夏文水 ( 1.武汉工业学院食品科学与工程学院，湖北 武汉 430023 ；2 .江南大学食品学院，江苏无锡 2 14 122) 摘 要：研究DA20 1-C 型大孔吸附树脂对鱼降压肽的脱盐工艺，并分析脱盐后的鱼降压肽的理化性质。结果表明， DA20 1-C 型大孔吸附树脂较佳脱盐工艺条件为上样质量浓度10mg/ml 、上样流速1.5BV/h 、解吸剂为70% 乙醇。鱼 降压肽经DA20 1-C 型大孔吸附树脂处理后其灰分由13 .89% 下降到3 .89% ，血管紧张素转化酶(A CE)抑制活性由 64 .35% 增加到95.00% 。脱盐后的鱼降压肽的分子质量分布范围在200～7000D 之间，其中在200～2000D 之间的 肽含量为8 1.7 % ；其在p H 2 ～12 的溶解度均为9 8% 以上，吸湿性跟大豆肽类似，吸湿率比尿素低，比甘油高。 关键词：鱼降压肽；大孔吸附树脂；血管紧张素转化酶(A C E ) 抑制活性；脱盐；理化性质 Desalting and Physico-chemical Properties of Antihypertensive Peptides from Grass Carp Protein with Macroporous Adsorption Resin 1 1 1,2 CHEN Ji-wang ，SUN Qin ，XIA Wen-shui (1. School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China； 2. School of Food Science and Technology, Jiangnan Unviersity, Wuxi 2 14 122, China) Abstract ：A certain amount of salts were introduced into fish antihypertensive peptides during the process of fish protein hydrolysis and 10% of ash in the final products was capable of influencing its activities and quality. The desalting technology of fish antihyperten sive peptides by DA20 1-C macroporous adsorption resin was investigated as well as its physic-chemical properties after desalting. The results indicated that the optimum condition for desalting was obtained as follows: loading sample concentration of 10 mg/ml, sampling flow rate of 1.5 BV/h and 70% alcohol as eluent. Under this condi