表达外源蛋白有利工具_毕赤酵母表达系统.pdfVIP

现代生物医学进展 Progress in Modern Biomedicine 2009 Vol.9 No.1 ·171 · - 表达外源蛋白的有利工具 毕赤酵母表达系统 1,2 1△ 李新丽 黄 瑾 1 2 新疆石河子832002 （新疆地方与民族高发病省部共建教育部重点实验室；新疆石河子大学医学院一附院检验科 ） 摘要：巴斯得毕赤酵母（Pichia pastoris）作为80 年代开发的一种表达系统，在外源蛋白的表达方面具有独特的优势，被广泛用于医 药领域和科学研究。本文介绍了酵母的生物学特性、常用菌株和表达载体的特点及其研究进展，并从外源基因本身序列的内在特 性和酵母发酵外源性因素两个方面，对影响外源蛋白表达的因素进行详细阐述。 关键词：毕赤酵母；外源蛋白；影响因素 Q816 A 1673-6273 2009 01-171-04 中图分类号： 文献标识码： 文章编号： （ ） Strong favorable tool for foreign protein expression -Pichia pastoris LI Xin -li1,2, HUANG Jin1△ （1 Coop eration Lab of Province Ministry of Education Key Laboratory of Xinj iang Endemic and Ethnic Disease, 2 Clinical laboratory of the first affiliated hosp ital of ShiHeZi University, Xinj iang Shihezi China, 832002） ABSTRACT: As a new development expression system in 80s, Pichia pastoris has unique advantage in expression of foreign pro- tein, and has been extensively used in medicine fields and scientific research. In this article, the biological characteristics of yeast, usual strains and plasmids and their research progress was reviewed, and the effect factors for foreign expression were described in detail from the following two aspects: one is internal characteristics of exogenous gene sequence; the other is external factor for yeast fermentation. Key words: Pichia pastoris; foreign protein; effect factors Chinese Library Classification: Q816 Document code: A Article ID: 1673-6273 （2009）01-171-04 随着现代分子生物学的快速发展，通过基因工程技术获得 lipolytica [2], 其中毕赤酵母和汉逊酵母是常用的外源蛋白表达 重组的功能