三氧化二砷抑制肝癌HepG2细胞侵袭转移和对RhoC基因表达的影响.pdfVIP

肿瘤防治研究 2008 年第 35 卷第 11 期 ·775 · 三氧化二砷抑制肝癌 Hep G2 细胞侵袭转移 及对 Rho C 基因表达的影响 冯觉平 ,黄 　涛 ,王亚萍 ,方 　静 ,李 　敏 ,孔庆志 Eff ect of Arsenic Trioxide on Invasion and Metastasis and RhoC Gene Expression of Hu man Hepatocarcinoma Cell s i n Vit r o F EN G J ueping , HU AN G Tao ,WAN G Yaping ,FAN G J ing ,L I Min , KON G Qingzhi D ep a rtment of Oncology , The P uai H osp i t al , Tongj i M e d ical col leg e , H uaz hong Uni vers i ty of S cience an d Technology , W u H an 430030 , Chi na. Corresp on d i ng A ut hor : H UA N G Tao , Em ai l :f engj uep i ng @163 . com Abstract :Objective 　 To evaluat e t he effect s of t he ar senic t rioxide (A s O ) on ant agonizing inva sion and 2 3 met ast a si s and to investigat e t he effect of A s2 O3 on Rho C gene exp ression in Human Hep atocarcinoma cell line Hep G2 . Methods 　A dherence abilit y ,Migration and inva sion of Hep G2 cell inhibit ed by A s O 2 3 were assessed by M T T and t ran swell t echnique . Exp ression level s of Rho CmRN A and p rot ein in Hep G2 cell were det ermined by rever se t ran scrip tionpolymera se chain reaction ( R TPCR) and West ern blot ,re sp ectively . Results 　The number of adhesion , migration and inva sion of Hep G2 cell s were significantly lower in A s O Group t han in t he cont rol group ( P 0 . 05) . A significant down - regulation of exp ression 2 3 of Rho C gene mRN A and p rot ein level s were ob served in Hep G2 cell s when 0 . 25 mg/ L A s O wa s given