姜黄素和干扰素_抑制慢性髓系白血病STAT5基因表达的研究.pdfVIP

C ST A T 5 * 1 1 v 2 1 1 1 1 1 陈伟红 陈 燕 任 澎 谷俊侠 薛克营 李新刚 吴 青 刘红利 1 , 4 30022 2 , 830001 C( IFN-C) ( CM L ) K562 ST A T 5 ( M T T ) ( RT- P CR ) ST A T 5 m RN A ST A T 5 IFN-C K 562 24 h , K 562 , ST AT 5 m R NA ST A T 5 IFN-C K 562 24 h , ST A T 5 m R N A ( P 0 0 1) IF N-C ST AT 5 ; C; ; , ST AT 5 R557 1 Inhibitory Eff ect of Curcumin and IFN-Con the Expression of STAT5 Gene in CML Ce 1 1 v 2 Chen Weihong , Chen Y an , Ren Peng et al 1Dep artment of H ematology, Union H osp ital, Tongj i Medical College, H uaz hong University of Science and Technology, uhan 430022 2Dep artment of Cardiology , Peop le. sH osp ital of Xinj iang Uyghur A utonomous Region, Urumqi 830001 Abstract Objective T o investig ate t he inhibito ry eff ect of cur cum in and IFN-Co n the ex pression of ST A T 5 g ene in chr o- nic my elo id leu kem ia ( CM L ) cell line ( K562 cell line) and the m echanism Methods Cell pro lif er atio n w as st udied by tetrazoli- um dye assay T he ex pression of ST A T 5 m RN A w as det ected by RT- PCR and th at of ST A T 5 protein by immunoblo tting and co nf o cal laser scanning m icr oscopy Resu ts When K562 cell s w er e treated w ith curcum in o r I FN-C f or 24 h , the ex pr ession level of ST AT 5 m RN A and pro tein w as dow n- reg ulated obviou sly T he ex pression level o f