山莨菪碱对大鼠急性肝损伤的保护作用及其机制.pdfVIP

658 1 8 11 10 11 邢卉春　赵龙凤　王守义 : : Wistar 18 3 : D (20 m g/ kg) 3 , ( NO) , NO( MDA) ( SOD) : ALT ( 666267) U AST ( 1 05 408) U TBIL ( 7. 225. 62) Lmol/ L ( 2 3526 8) U, ( 3 54 717) U ( 20. 71 5. 2) Lmol/ L ,P 0.01; NO , MDA SOD (P 0.01) : , NO , Protective effect of anisodamine on acute liver injury in rats and its potential mechanism X ing H uichun,Zhao Longf eng, Wang Shouyi.Dep artment of Inf ection Disease, The First H osp ital of Shanx i M edical niversity, Taiyuan 030001 AbstractObjective: To study the protective effect of anisodamine on acute liver injury and its po- tential mechanism . Methods: Rats were injected int raperitoneally w ith D Galactosamine ( D GalN, 0. 6 g/ kg) plus lipopolysaccharide ( LPS, 0. 1 mg/ kg) to induce acute liver injury. Anim als were ran- domly divided into normal control group, liver injury group and treatment group ( pret reatm ent w it h anisodam ine 20 mg/ kg) . Tw elve hours later, serum and liver tissues w ere collected to measure liver function parameters, lipid peroxidation (malondialdehyde, M DA) , nitric oxide ( NO) levels and superox- ide dism ut ase ( SOD) activity , and pathology change of liver w as also observed. Results: Serum alanine aminotransferase ALT ( 666267) U , aspartate aminotransferase AST ( 1 05 408) U and total bilirubin TBIL (7. 225. 62) Lmol/ L in anisodamine treat ed group w ere significantly low er than t hose in animals w ithout treatment ( 2 3526 8) U, ( 3 54 717) U, ( 20.715. 2) Lmol/ L respec- tively , all P 0.01. Pretreatment w ith anisodamine, it obviously reduced the elevation of NO levels both in serum and t issue compared t o liver injury group, and similar changes were also observed in tis- sue MDA levels,w hile tissue SOD act ivities w ere markedly increa