川芎嗪注射液对小鼠缺血再灌注心肌的保护作用_李萍.pdfVIP

# 32# 2006 26 1 Chin Hosp Pharm J, 2006 Jan, V ol26, No. 01 1 2 2 1 1 1 李萍 ,李洪, 贺冬林 ,赵燕 , 熊凡 , 富青 ( 1. , 430 064; 2 . , , 43 007 9) [] : 观察川芎嗪注射液( LGT)对心肌缺血再灌注的影响并 讨其作用机制: 将小鼠随机分为 4 组, 以 40% 川芎 - 1 嗪注射液 0. 25 mL# ( 10 g) 灌胃处理, 1周后腹腔注射垂体后叶素( Pit)和硝酸甘油(N it), 复制心肌缺血再灌注模型,观察小鼠 标准Ò导联心电图变化; 心肌组织一氧化氮合酶(NOS)超氧化物歧化酶( SO )活性,脂质过氧化物丙二醛(M A)含量;血清肌酸 激酶(CK)活性: 缺血再灌注小鼠心电图 J点发生明显偏移, LGT 预处理小鼠心电图 J点变化幅度明显减少, 心肌组织 NOSSO 活性显著增加, M A的含量及血清 CK活性显著降低(P 0. 01): 川芎嗪注射液能保护心脏, 减轻心肌缺血再 灌注引发的损伤;其作用机制可能与增加缺血再灌注心肌组织 NOSSO 活性, 减少M A含量和血清 CK活性有关 [] 川芎嗪注射液; 心肌缺血再灌注; 一氧化氮合酶; 超氧化物歧化酶; 丙二醛 [] R965 [] A [] 1001-52 13 (2006) 01-0032-03 P rotective effect of ligustrazine injection on the ischem ia and re-perfusion myocardium 1 2 2 1 1 1 LI Ping , LIHong, HE ang-ling , ZHAO Yan, XIONG Fan, FU Qing ( 1. HubeiCollege of Traditional Chinese M ed-i cine, HubeiW uhan 430 064, China; 2. HubeiCancerHospital, HubeiWuhan 4 300 79, China) ABSTRACT: OB ECTIVE To observe the effect of ligustrazine injection on themyocardial ischem ia-re-perfusion injury in rats and to study itsmechanism of action. M ETHODS M icewere random ly divided into four groups and treatedw ith 4 0% ligustrazine injec- - 1 - 1 tion. Oneweek later, the micewere injected with pituitrin( 30 U# kg ) and nitroglycerin( 10 mg# kg , 3 0 m in later) intraperitoneally to remodelmyocardial ischem ic and re-perfusion injury. ECG was recorded. 3 h later, the activities of nitric oxide synthase(NOS), su- peroxide dismutase( SO ) and the content ofmalondiadehyde(M A) in themyocardium and creatine kinase ( CK) activity of serum were examined. RESULTS L igustrazine reduced changes in J spot in ECG, increased activity ofNOS and SO , inhi