帕金森病MPTP模型小鼠两种行为学检测方法的比较研究.pdfVIP

2009 30 15 # 1809 # # # M PT P 李丽波 王玉祥 = 在帕金森病M PT P 模型小鼠对爬杆法( po le test) 和转棒法( r otar od test) 两种行为 学检测方法进行 较研究 C57/ BL 小鼠腹腔注射 1- 甲基- 4 - 苯基1, 2 , 3, 6- 四氢吡啶 ( M PT P) 30 mg / kg , 每天1 次, 连续5 天, 于第1234 5 天分别用爬杆法和转棒法进行行为学检测 爬杆法的调头时间和爬下时间与小鼠的适应性行为有关, 且其评分标准易掺杂主观因素 而转棒 法是小鼠的被动运动, 潜伏期和掉落次数均为客观的观察指标 与爬杆法相 , 转棒法是更为客 观的帕金森模型小鼠的行为学检测方法 = 1- 甲基- 4- 苯基1, 2 , 3, 6- 四氢吡啶( M P T P ) 帕金森病 行为学 爬杆法 转棒 法 A comparative study on two ethologyical methods in MPTP model mice of Parkinsons disease LI L i - bo , et al . ( Qiqih ar M edical U niver sity, Heilongjiang 16100 6, China) = Abstract Objective T o investig ate t he diff erence betw een pole test and rota rod test in M PT P model m ice of Parkinson s disease. Methods C57/ BL m ice w er e int rap erito neally inj ect ed ( ip) 1- M eth- yl - 4- pheny l- 1, 2, 3, 6- tetr ahy dr o - pyr idine( M PT P) 30mg/ kg once daily f or co nsecut ive f iv e days and their behav io r w as ex am ined in po le test and r otaro d test on the 1st , 2nd , 3rd , 4th and 5th day. Results Subj ect ive f actor w as inclined to be invo lv ed in pole test indicato rs in r otar od t est w ere mo re objective. Conclusions Rota rod test is a mor e o bj ective etho log y ical m etho d th an po le test f o r M PT P mo del m ice of P arkin sons disease. = Key words Parkin son s disease M PT P Etholog y P ole test Ro taro d test ( Parkinson s disease, P D) 2. 5 cm , , 10 [ 5] , / : , , - , 1 cm , 60 cm 1. 1. 3 C57/ BL , 22 ? 2 g , , 1-