1-s2.0-S0925400512014281-main.pdfVIP

Sensors and Actuators B 178 (2013) 525–531 Contents lists available at SciVerse ScienceDirect Sensors and Actuators B: Chemical j o u rn a l h o me p ag e: w w w. elsev /locate/snb A benzoxazine–hemicyanine based probe for the colorimetric and ratiometric detection of biothiols Xiao-Dong Liu a , Ru Sun a,∗ , Ying Xu b , Yu-Jie Xu b , Jian-Feng Ge a,∗ , Jian-Mei Lu a,c,∗ a Key Laboratory of Organic Synthesis ofJiangsu Province, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou 215123, China b School of Radiation Medicine and Protection, Medicine College of Soochow University, Suzhou 215123, China c Key Laboratory of Functional Materials for Environment Protection of Suzhou City, Soochow University, Suzhou 215123, China a r t i c l e i n f o a b s t r a c t Article history: Aliphatic biothiols, including cysteine, homocysteine, and glutathione, are associated with a wide range Received 16 November 2012 of biological functions. Hence, the selective detection of intracellular thiols has significance for investi- Received in revised form gating cellular function. Ratiometric fluorescent probes are very useful in the biological sciences because 17 December 2012 they allow measurement at two emission wavelengths, providing a built-in correction for environmental Accepted 29 December 2012 effects. In this study, a benzoxazine–hemicyanine based probe (1) was synthesized and characterized. Available online 4 January 2013