重组人EPO对rhIL_6诱导H_省略_cidinmRNA表达的抑制作用_孟婵.pdfVIP

Jour al o fExpermi e tal Hem atology 2008; 16( 6): 14 13- 1417 # 14 13# ( Article ID): 1009- 2137( 2008) 06- 14 13- 05 # 论著# EPO rhIL26H epG2 H epcidin m RNA 1 1 , , , , , 中国医学科学院北京协和医院血液内科, 北京 100730; 1 中国医学科学院 基础医学研究所细胞学中心, 北京 100730 EPOH epcid i rhEPO 。 H epG2 rhIL26、rhEPO 。 mRNA, RT2PCR , H epcidi G3PDH mRNA; H epcid i 。: rhIL26 H epG2H epcid i mRNA , rhEPO IL26 H epcidi mRNA, rhEPO H epG2H epcid i 。 : IL26H epG2H epcid i mRNA, 、 , rhEPO 。 H epcid i ; ; EPO; IL26; H epG2; R 556 A Inhib ition E ffect of rhEPO on rhIL62induced H epcidin mRNA Expression in H epG2 CellL ines and Hum an Prim ary H epatocytes 1 1 MENG Chan, ZHOU Dao2B in, ZHAO Yong2Q iang, WANG Xuan, GU B ei , LI U X iu 2Q in 1 D eparmt e t ofH ematology, P eki g U io M edica l College Hosp ita l, Beiji g 100730, Ch i a; Ce tre of Cytology, I stitute ofBa sic M edica l Scie ces, Ch i ese Academ y ofM edical Scie ces, Beiji g 100730, Chi a Abstract Th is study was ami ed to i vestigate the effect of rhIL26 a d rhEPO o hepcid i mRNA expressio i H epG2 cells a d hum a prmi ary hepatocytes, a d m echa ism of rhEPO i treamt e t of a em ia of chro ic disease ( ACD ). TheHepG 2 cells a d hum a prmi ary hepatocytesw ere culturedw ith m edium co tai i g d iffere t co ce tratio s of rh IL26 a d rhEPO for a certai tmi e, the mRNA was isolated a d its R